Griffitts:Protocols
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==RNA== | ==RNA== | ||
| - | *Trizol purification (bacterial) | + | * [[Griffitts:Trizol purification|Trizol purification (bacterial)]] |
| - | *DNAse treatment | + | * [[Griffitts:DNAse treatment|DNAse treatment]] |
| - | *Reverse transcription | + | * [[Griffitts:Reverse transcription|Reverse transcription]] |
| - | *5'RACE | + | * [[Griffitts:5' RACE|5' RACE]] |
| + | |||
==Protein== | ==Protein== | ||
* [[Griffitts:Gel recipes|Gel recipes]] | * [[Griffitts:Gel recipes|Gel recipes]] | ||
Revision as of 15:10, 13 February 2008
Home Research Lab Members Protocols Publications Internal
Contents |
Recipes
DNA
PCR
- PCR primers
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Sequencing prep
DNA prep
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
