Griffitts:Protocols: Difference between revisions
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* [[Griffitts:Freezing bacteria|Freezing procedure]] | * [[Griffitts:Freezing bacteria|Freezing procedure]] | ||
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]] | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]] | ||
* [[Griffitts: | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] | ||
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
Revision as of 14:05, 11 April 2008
Recipes
DNA
PCR
- PCR primers
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Sequencing prep
DNA prep
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
