Griffitts:Protocols: Difference between revisions
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===PCR=== | ===PCR=== | ||
* [[Griffitts:PCR primers|PCR primers]] | * [[Griffitts:PCR primers|PCR primers]] | ||
* [[Griffitts: | * [[Griffitts:PCR|Standard PCR]] | ||
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | * [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | ||
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | ||
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | * [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | ||
* [[Griffitts:DNA sequencing|Sequencing prep]] | * [[Griffitts:DNA sequencing|Sequencing prep]] | ||
===DNA prep=== | ===DNA prep=== | ||
* [[Griffitts:Miniprep|Qiagen Miniprep]] | * [[Griffitts:Miniprep|Qiagen Miniprep]] | ||
Revision as of 10:33, 3 July 2008
Recipes
DNA
PCR
DNA prep
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
