Griffitts:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
m (→Bacteria) |
||
| (43 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
{{Template:Griffitts}} | {{Template:Griffitts}} | ||
==Recipes== | ==Recipes== | ||
*Common buffers | * [[Griffitts:Common buffers|Common buffers]] | ||
*Common media | * [[Griffitts:Common media|Common media]] | ||
* [[Griffitts:Stock solutions|Stock solutions]] | |||
==DNA== | ==DNA== | ||
*[[Griffitts:Miniprep|Qiagen Miniprep]] | * [[Griffitts:Phylogenetics|Phylogenetics]] | ||
*Low-copy plasmid prep | |||
*[[Griffitts: | ===PCR=== | ||
* | * [[Griffitts:PCR primers|PCR primers]] | ||
* | * [[Griffitts:PCR|Standard PCR]] | ||
* | * [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | ||
*Restriction digest | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | ||
*In-gel ligation | * [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | ||
* [[Griffitts:DNA sequencing|Sequencing prep]] | |||
===DNA prep=== | |||
* [[Griffitts:Miniprep|Qiagen Miniprep]] | |||
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | |||
* [[Griffitts:Midiprep|Nucleobond Midiprep]] | |||
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | |||
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | |||
* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | |||
* [[Griffitts:Restriction digest|Restriction digest]] | |||
* [[Griffitts:In-gel ligation|In-gel ligation]] | |||
==RNA== | ==RNA== | ||
*Trizol purification (bacterial) | * [[Griffitts:Trizol purification|Trizol RNA purification (bacterial)]] | ||
*DNAse treatment | * [[Griffitts:DNAse treatment|DNAse treatment]] | ||
*Reverse transcription | * [[Griffitts:Reverse transcription|Reverse transcription]] | ||
*5'RACE | * [[Griffitts:5' RACE|5' RACE]] | ||
==Protein== | ==Protein== | ||
*Gel recipes | * [[Griffitts:Gel recipes|Gel recipes]] | ||
*Sample buffer | * [[Griffitts:Common buffers|Sample buffer]] | ||
*Bacterial lysis | * [[Griffitts:Bacterial lysis|Bacterial lysis]] | ||
*6His purification | * [[Griffitts:6His purification|6His purification]] | ||
*MBP purification | * [[Griffitts:MBP purification|MBP purification]] | ||
==Bacteria== | ==Bacteria== | ||
*Media | * [[Griffitts:Common media#Bacterial Media and Additives|Media]] | ||
*Antibiotics | * [[Griffitts:Stock solutions#Antibiotics|Antibiotics]] | ||
*Tips on streaking/culturing | * [[Griffitts:Culture tips|Tips on streaking/culturing]] | ||
*Freezing | * [[Griffitts:Culture tips#Freeze Down|Freezing procedure]] | ||
*Genomic DNA prep | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | ||
*RNA prep (Trizol) | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] | ||
* | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
*Triparental Mating | * [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]] | ||
*Transduction | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* [[Griffitts:Transduction|Transduction]] | |||
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]] | |||
** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]] | |||
* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]] | |||
** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | |||
* [[Griffitts:LPS Analysis|LPS Analysis]] | |||
==Plants== | ==Plants== | ||
*Media | * [[Griffitts:Common media#Plant Media and Additives|Media]] | ||
*Seed scarification | * [[Griffitts:Conetainers|Conetainer Rack Protocol]] | ||
*Tips for growth/seed storage | * [[Griffitts:Seed scarification and germination|Seed scarification and germination]] | ||
*DNA prep | * [[Griffitts:Plant tips|Tips for growth/seed storage]] | ||
*RNA prep | * [[Griffitts:Plant DNA preparation|DNA prep]] | ||
*Seed Storage | * [[Griffitts:Plant RNA preparation|RNA prep]] | ||
*Microscopy | * [[Griffitts:Seed Storage|Seed Storage]] | ||
* [[Griffitts:Microscopy|Microscopy]] | |||
* [[Griffitts:Nodule Recovery|Nodule Bacteria Recovery]] | |||
* [[Griffitts:Nodule Fixation|Nodule Fixation/Embedding]] | |||
* [[Griffitts:Nodule Staining|LacZ Staining of Nodules]] | |||
Revision as of 10:29, 16 May 2011
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
