Griffitts:Protocols
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{{Template:Griffitts}} | {{Template:Griffitts}} | ||
==Recipes== | ==Recipes== | ||
| - | *[[Griffitts:Common buffers|Common buffers]] | + | * [[Griffitts:Common buffers|Common buffers]] |
| - | *[[Griffitts:Common media|Common media]] | + | * [[Griffitts:Common media|Common media]] |
| + | * [[Griffitts:Stock solutions|Stock solutions]] | ||
==DNA== | ==DNA== | ||
| - | *[[Griffitts:Miniprep|Qiagen Miniprep]] | + | * [[Griffitts:Phylogenetics|Phylogenetics]] |
| - | *Low-copy plasmid prep | + | |
| - | *[[Griffitts: | + | ===PCR=== |
| - | * | + | * [[Griffitts:PCR primers|PCR primers]] |
| - | * | + | * [[Griffitts:PCR|Standard PCR]] |
| - | * | + | * [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] |
| - | *Restriction digest | + | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] |
| - | *In-gel ligation | + | * [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] |
| + | * [[Griffitts:DNA sequencing|Sequencing prep]] | ||
| + | |||
| + | ===DNA prep=== | ||
| + | * [[Griffitts:Miniprep|Qiagen Miniprep]] | ||
| + | * [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | ||
| + | * [[Griffitts:Midiprep|Nucleobond Midiprep]] | ||
| + | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | ||
| + | * [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | ||
| + | * [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | ||
| + | * [[Griffitts:Restriction digest|Restriction digest]] | ||
| + | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
| + | |||
==RNA== | ==RNA== | ||
| - | *Trizol purification (bacterial) | + | * [[Griffitts:Trizol purification|Trizol RNA purification (bacterial)]] |
| - | *DNAse treatment | + | * [[Griffitts:DNAse treatment|DNAse treatment]] |
| - | *Reverse transcription | + | * [[Griffitts:Reverse transcription|Reverse transcription]] |
| - | *5'RACE | + | * [[Griffitts:5' RACE|5' RACE]] |
| + | |||
==Protein== | ==Protein== | ||
| - | *Gel recipes | + | * [[Griffitts:Gel recipes|Gel recipes]] |
| - | *Sample buffer | + | * [[Griffitts:Common buffers|Sample buffer]] |
| - | *Bacterial lysis | + | * [[Griffitts:Bacterial lysis|Bacterial lysis]] |
| - | *6His purification | + | * [[Griffitts:6His purification|6His purification]] |
| - | *MBP purification | + | * [[Griffitts:MBP purification|MBP purification]] |
| + | |||
==Bacteria== | ==Bacteria== | ||
| - | *[[Griffitts:Common media#Bacterial Media and Additives|Media]] | + | * [[Griffitts:Common media#Bacterial Media and Additives|Media]] |
| - | *Antibiotics | + | * [[Griffitts:Stock solutions#Antibiotics|Antibiotics]] |
| - | *Tips on streaking/culturing | + | * [[Griffitts:Culture tips|Tips on streaking/culturing]] |
| - | *Freezing | + | * [[Griffitts:Culture tips#Freeze Down|Freezing procedure]] |
| - | *Genomic DNA prep | + | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] |
| - | *RNA prep (Trizol) | + | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] |
| - | * | + | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] |
| - | *Triparental Mating | + | * [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]] |
| - | *Transduction | + | * [[Griffitts:Triparental Mating|Triparental Mating]] |
| + | * [[Griffitts:Transduction|Transduction]] | ||
| + | * [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]] | ||
| + | ** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]] | ||
| + | * [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]] | ||
| + | ** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ||
| + | * [[Griffitts:LPS Analysis|LPS Analysis]] | ||
==Plants== | ==Plants== | ||
| - | *Media | + | * [[Griffitts:Common media#Plant Media and Additives|Media]] |
| - | *Seed scarification | + | * [[Griffitts:Conetainers|Conetainer Rack Protocol]] |
| - | *Tips for growth/seed storage | + | * [[Griffitts:Seed scarification and germination|Seed scarification and germination]] |
| - | *DNA prep | + | * [[Griffitts:Plant tips|Tips for growth/seed storage]] |
| - | *RNA prep | + | * [[Griffitts:Plant DNA preparation|DNA prep]] |
| - | *Seed Storage | + | * [[Griffitts:Plant RNA preparation|RNA prep]] |
| - | *Microscopy | + | * [[Griffitts:Seed Storage|Seed Storage]] |
| + | * [[Griffitts:Microscopy|Microscopy]] | ||
| + | * [[Griffitts:Nodule Recovery|Nodule Bacteria Recovery]] | ||
| + | * [[Griffitts:Nodule Fixation|Nodule Fixation/Embedding]] | ||
| + | * [[Griffitts:Nodule Staining|LacZ Staining of Nodules]] | ||
Revision as of 13:29, 16 May 2011
Home Research Lab Members Protocols Publications Internal
Contents |
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
