Griffitts:Protocols

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{{Template:Griffitts}}
{{Template:Griffitts}}
==Recipes==
==Recipes==
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*[[Griffitts:Common buffers|Common buffers]]
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* [[Griffitts:Common buffers|Common buffers]]
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*[[Griffitts:Common media|Common media]]
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* [[Griffitts:Common media|Common media]]
 +
* [[Griffitts:Stock solutions|Stock solutions]]
==DNA==
==DNA==
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*[[Griffitts:Miniprep|Qiagen Miniprep]]
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* [[Griffitts:Phylogenetics|Phylogenetics]]
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*Low-copy plasmid prep
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*[[Griffitts:TaqPCR|Standard PCR]]
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===PCR===
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*Pfx PCR
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* [[Griffitts:PCR primers|PCR primers]]
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*Overlap-extension PCR
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* [[Griffitts:PCR|Standard PCR]]
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*Arbitrary PCR
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* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
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*Restriction digest
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* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
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*In-gel ligation
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* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
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* [[Griffitts:DNA sequencing|Sequencing prep]]
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 +
===DNA prep===
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* [[Griffitts:Miniprep|Qiagen Miniprep]]
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* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
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* [[Griffitts:Midiprep|Nucleobond Midiprep]]
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* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]]
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* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
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* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]]
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* [[Griffitts:Restriction digest|Restriction digest]]
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* [[Griffitts:In-gel ligation|In-gel ligation]]
 +
 
==RNA==
==RNA==
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*Trizol purification (bacterial)
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* [[Griffitts:Trizol purification|Trizol RNA purification (bacterial)]]
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*DNAse treatment
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* [[Griffitts:DNAse treatment|DNAse treatment]]
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*Reverse transcription
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* [[Griffitts:Reverse transcription|Reverse transcription]]
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*5'RACE
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* [[Griffitts:5' RACE|5' RACE]]
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==Protein==
==Protein==
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*[[Griffitts:Gel recipes|Gel recipes]]
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* [[Griffitts:Gel recipes|Gel recipes]]
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*Sample buffer
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* [[Griffitts:Common buffers|Sample buffer]]
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*Bacterial lysis
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* [[Griffitts:Bacterial lysis|Bacterial lysis]]
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*6His purification (6His)
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* [[Griffitts:6His purification|6His purification]]
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*MBP purification (MBP)
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* [[Griffitts:MBP purification|MBP purification]]
==Bacteria==
==Bacteria==
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*[[Griffitts:Common media#Bacterial Media and Additives|Media]]
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* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
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*Antibiotics
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* [[Griffitts:Stock solutions#Antibiotics|Antibiotics]]
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*Tips on streaking/culturing
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* [[Griffitts:Culture tips|Tips on streaking/culturing]]
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*Freezing Procedure
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* [[Griffitts:Culture tips#Freeze Down|Freezing procedure]]
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*Genomic DNA prep
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* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]]
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*RNA prep (Trizol)
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* [[Griffitts:Trizol purification|RNA prep (Trizol)]]
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*[[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
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* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
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*Triparental Mating
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* [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]]
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*Transduction
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* [[Griffitts:Triparental Mating|Triparental Mating]]
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* [[Griffitts:Transduction|Transduction]]
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* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]]
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** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]]
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* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]]
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** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]]
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* [[Griffitts:LPS Analysis|LPS Analysis]]
==Plants==
==Plants==
* [[Griffitts:Common media#Plant Media and Additives|Media]]
* [[Griffitts:Common media#Plant Media and Additives|Media]]
 +
* [[Griffitts:Conetainers|Conetainer Rack Protocol]]
* [[Griffitts:Seed scarification and germination|Seed scarification and germination]]
* [[Griffitts:Seed scarification and germination|Seed scarification and germination]]
* [[Griffitts:Plant tips|Tips for growth/seed storage]]
* [[Griffitts:Plant tips|Tips for growth/seed storage]]
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* [[Griffitts:DNA preparation|DNA prep]]
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* [[Griffitts:Plant DNA preparation|DNA prep]]
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* [[Griffitts:RNA preparation|RNA prep]]
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* [[Griffitts:Plant RNA preparation|RNA prep]]
* [[Griffitts:Seed Storage|Seed Storage]]
* [[Griffitts:Seed Storage|Seed Storage]]
* [[Griffitts:Microscopy|Microscopy]]
* [[Griffitts:Microscopy|Microscopy]]
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* [[Griffitts:Nodule Recovery|Nodule Bacteria Recovery]]
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* [[Griffitts:Nodule Fixation|Nodule Fixation/Embedding]]
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* [[Griffitts:Nodule Staining|LacZ Staining of Nodules]]

Revision as of 12:29, 16 May 2011

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Contents

Recipes

DNA

PCR

DNA prep

RNA

Protein

Bacteria

Plants

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