Griffitts:Protocols
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==DNA== | ==DNA== | ||
| - | * [[Griffitts: | + | * [[Griffitts:Phylogenetics|Phylogenetics]] |
| - | + | ||
| + | ===PCR=== | ||
* [[Griffitts:PCR primers|PCR primers]] | * [[Griffitts:PCR primers|PCR primers]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:PCR|Standard PCR]] |
| - | + | ||
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | * [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | ||
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | ||
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | * [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | ||
| + | * [[Griffitts:DNA sequencing|Sequencing prep]] | ||
| + | |||
| + | ===DNA prep=== | ||
| + | * [[Griffitts:Miniprep|Qiagen Miniprep]] | ||
| + | * [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | ||
| + | * [[Griffitts:Midiprep|Nucleobond Midiprep]] | ||
| + | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | ||
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | * [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | ||
| + | * [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | ||
* [[Griffitts:Restriction digest|Restriction digest]] | * [[Griffitts:Restriction digest|Restriction digest]] | ||
* [[Griffitts:In-gel ligation|In-gel ligation]] | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
| - | |||
==RNA== | ==RNA== | ||
| - | *Trizol purification (bacterial) | + | * [[Griffitts:Trizol purification|Trizol RNA purification (bacterial)]] |
| - | *DNAse treatment | + | * [[Griffitts:DNAse treatment|DNAse treatment]] |
| - | *Reverse transcription | + | * [[Griffitts:Reverse transcription|Reverse transcription]] |
| - | *5'RACE | + | * [[Griffitts:5' RACE|5' RACE]] |
| + | |||
==Protein== | ==Protein== | ||
| - | *[[Griffitts:Gel recipes|Gel recipes]] | + | * [[Griffitts:Gel recipes|Gel recipes]] |
| - | *Sample buffer | + | * [[Griffitts:Common buffers|Sample buffer]] |
| - | *Bacterial lysis | + | * [[Griffitts:Bacterial lysis|Bacterial lysis]] |
| - | *6His purification | + | * [[Griffitts:6His purification|6His purification]] |
| - | *MBP purification | + | * [[Griffitts:MBP purification|MBP purification]] |
==Bacteria== | ==Bacteria== | ||
* [[Griffitts:Common media#Bacterial Media and Additives|Media]] | * [[Griffitts:Common media#Bacterial Media and Additives|Media]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:Stock solutions#Antibiotics|Antibiotics]] |
* [[Griffitts:Culture tips|Tips on streaking/culturing]] | * [[Griffitts:Culture tips|Tips on streaking/culturing]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:Culture tips#Freeze Down|Freezing procedure]] |
| - | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]] | + | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] |
| - | * [[Griffitts: | + | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] |
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
| + | * [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]] | ||
* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* [[Griffitts:Transduction|Transduction]] | * [[Griffitts:Transduction|Transduction]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]] |
| - | * [[Griffitts: | + | ** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]] |
| + | * [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]] | ||
| + | ** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ||
| + | * [[Griffitts:LPS Analysis|LPS Analysis]] | ||
==Plants== | ==Plants== | ||
* [[Griffitts:Common media#Plant Media and Additives|Media]] | * [[Griffitts:Common media#Plant Media and Additives|Media]] | ||
| + | * [[Griffitts:Conetainers|Conetainer Rack Protocol]] | ||
* [[Griffitts:Seed scarification and germination|Seed scarification and germination]] | * [[Griffitts:Seed scarification and germination|Seed scarification and germination]] | ||
* [[Griffitts:Plant tips|Tips for growth/seed storage]] | * [[Griffitts:Plant tips|Tips for growth/seed storage]] | ||
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* [[Griffitts:Seed Storage|Seed Storage]] | * [[Griffitts:Seed Storage|Seed Storage]] | ||
* [[Griffitts:Microscopy|Microscopy]] | * [[Griffitts:Microscopy|Microscopy]] | ||
| + | * [[Griffitts:Nodule Recovery|Nodule Bacteria Recovery]] | ||
| + | * [[Griffitts:Nodule Fixation|Nodule Fixation/Embedding]] | ||
| + | * [[Griffitts:Nodule Staining|LacZ Staining of Nodules]] | ||
Revision as of 13:29, 16 May 2011
Home Research Lab Members Protocols Publications Internal
Contents |
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
