Griffitts:Protocols

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m (RNA)
m (Bacteria)
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==DNA==
==DNA==
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* [[Griffitts:Phylogenetics|Phylogenetics]]
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===PCR===
===PCR===
* [[Griffitts:PCR primers|PCR primers]]
* [[Griffitts:PCR primers|PCR primers]]
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* [[Griffitts:TaqPCR|Standard PCR]]
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* [[Griffitts:PCR|Standard PCR]]
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* [[Griffitts:Pfx PCR|Pfx PCR]]
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* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
* [[Griffitts:DNA sequencing|Sequencing prep]]
* [[Griffitts:DNA sequencing|Sequencing prep]]
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===DNA prep===
===DNA prep===
* [[Griffitts:Miniprep|Qiagen Miniprep]]
* [[Griffitts:Miniprep|Qiagen Miniprep]]
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
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* [[Griffitts:Midiprep|Nucleobond Midiprep]]
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* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]]
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
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* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:In-gel ligation|In-gel ligation]]
* [[Griffitts:In-gel ligation|In-gel ligation]]
==RNA==
==RNA==
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* [[Griffitts:Trizol purification|Trizol purification (bacterial)]]
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* [[Griffitts:Trizol purification|Trizol RNA purification (bacterial)]]
* [[Griffitts:DNAse treatment|DNAse treatment]]
* [[Griffitts:DNAse treatment|DNAse treatment]]
* [[Griffitts:Reverse transcription|Reverse transcription]]
* [[Griffitts:Reverse transcription|Reverse transcription]]
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==Bacteria==
==Bacteria==
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
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* [[Griffitts:Stock_solutions#Antibiotics|Antibiotics]]
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* [[Griffitts:Stock solutions#Antibiotics|Antibiotics]]
* [[Griffitts:Culture tips|Tips on streaking/culturing]]
* [[Griffitts:Culture tips|Tips on streaking/culturing]]
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* [[Griffitts:Freezing bacteria|Freezing procedure]]
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* [[Griffitts:Culture tips#Freeze Down|Freezing procedure]]
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* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]]
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* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]]
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* [[Griffitts:Bacterial RNA preparation|RNA prep (Trizol)]]
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* [[Griffitts:Trizol purification|RNA prep (Trizol)]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
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* [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]]
* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Transduction|Transduction]]
* [[Griffitts:Transduction|Transduction]]
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]]
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]]
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** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]]
* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]]
* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]]
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** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]]
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* [[Griffitts:LPS Analysis|LPS Analysis]]
==Plants==
==Plants==
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* [[Griffitts:Seed Storage|Seed Storage]]
* [[Griffitts:Seed Storage|Seed Storage]]
* [[Griffitts:Microscopy|Microscopy]]
* [[Griffitts:Microscopy|Microscopy]]
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* [[Griffitts:Nodule Recovery|Nodule Bacteria Recovery]]
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* [[Griffitts:Nodule Fixation|Nodule Fixation/Embedding]]
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* [[Griffitts:Nodule Staining|LacZ Staining of Nodules]]

Revision as of 12:29, 16 May 2011

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