Griffitts:Protocols
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==DNA== | ==DNA== | ||
| + | * [[Griffitts:Phylogenetics|Phylogenetics]] | ||
| + | |||
===PCR=== | ===PCR=== | ||
* [[Griffitts:PCR primers|PCR primers]] | * [[Griffitts:PCR primers|PCR primers]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:PCR|Standard PCR]] |
| - | + | ||
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | * [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]] | ||
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | * [[Griffitts:Arbitrary PCR|Arbitrary PCR]] | ||
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | * [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]] | ||
* [[Griffitts:DNA sequencing|Sequencing prep]] | * [[Griffitts:DNA sequencing|Sequencing prep]] | ||
| + | |||
===DNA prep=== | ===DNA prep=== | ||
* [[Griffitts:Miniprep|Qiagen Miniprep]] | * [[Griffitts:Miniprep|Qiagen Miniprep]] | ||
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | * [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | ||
| + | * [[Griffitts:Midiprep|Nucleobond Midiprep]] | ||
| + | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | ||
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | * [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | ||
| + | * [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | ||
* [[Griffitts:Restriction digest|Restriction digest]] | * [[Griffitts:Restriction digest|Restriction digest]] | ||
* [[Griffitts:In-gel ligation|In-gel ligation]] | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
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==Bacteria== | ==Bacteria== | ||
* [[Griffitts:Common media#Bacterial Media and Additives|Media]] | * [[Griffitts:Common media#Bacterial Media and Additives|Media]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:Stock solutions#Antibiotics|Antibiotics]] |
* [[Griffitts:Culture tips|Tips on streaking/culturing]] | * [[Griffitts:Culture tips|Tips on streaking/culturing]] | ||
| - | * [[Griffitts: | + | * [[Griffitts:Culture tips#Freeze Down|Freezing procedure]] |
| - | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]] | + | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] |
* [[Griffitts:Trizol purification|RNA prep (Trizol)]] | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] | ||
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
| + | * [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]] | ||
* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* [[Griffitts:Transduction|Transduction]] | * [[Griffitts:Transduction|Transduction]] | ||
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]] | * [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]] | ||
| + | ** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]] | ||
* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]] | * [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]] | ||
| + | ** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ||
| + | * [[Griffitts:LPS Analysis|LPS Analysis]] | ||
==Plants== | ==Plants== | ||
Revision as of 13:29, 16 May 2011
Home Research Lab Members Protocols Publications Internal
Contents |
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
