Griffitts:Protocols: Difference between revisions
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==DNA== | ==DNA== | ||
* [[Griffitts:Phylogenetics|Phylogenetics]] | |||
===PCR=== | ===PCR=== | ||
* [[Griffitts:PCR primers|PCR primers]] | * [[Griffitts:PCR primers|PCR primers]] | ||
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* [[Griffitts:Trizol purification|RNA prep (Trizol)]] | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] | ||
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
* [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]] | |||
* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* [[Griffitts:Transduction|Transduction]] | * [[Griffitts:Transduction|Transduction]] | ||
Revision as of 10:29, 16 May 2011
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
