Griffitts:Protocols: Difference between revisions

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==DNA==
==DNA==
* [[Griffitts:Phylogenetics|Phylogenetics]]
===PCR===
===PCR===
* [[Griffitts:PCR primers|PCR primers]]
* [[Griffitts:PCR primers|PCR primers]]
* [[Griffitts:TaqPCR|Standard PCR]]
* [[Griffitts:PCR|Standard PCR]]
* [[Griffitts:Pfx PCR|Pfx PCR]]
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
* [[Griffitts:Overlap-extension PCR|Overlap-extension PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:Arbitrary PCR|Arbitrary PCR]]
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
* [[Griffitts:PCR clean-up|PCR clean-up (Qiagen)]]
* [[Griffitts:DNA sequencing|Sequencing prep]]
* [[Griffitts:DNA sequencing|Sequencing prep]]
===DNA prep===
===DNA prep===
* [[Griffitts:Miniprep|Qiagen Miniprep]]
* [[Griffitts:Miniprep|Qiagen Miniprep]]
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]]
* [[Griffitts:Midiprep|Nucleobond Midiprep]]
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]]
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]]
* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:Restriction digest|Restriction digest]]
* [[Griffitts:In-gel ligation|In-gel ligation]]
* [[Griffitts:In-gel ligation|In-gel ligation]]


==RNA==
==RNA==
*Trizol purification (bacterial)
* [[Griffitts:Trizol purification|Trizol RNA purification (bacterial)]]
*DNAse treatment
* [[Griffitts:DNAse treatment|DNAse treatment]]
*Reverse transcription
* [[Griffitts:Reverse transcription|Reverse transcription]]
*5'RACE
* [[Griffitts:5' RACE|5' RACE]]
 
==Protein==
==Protein==
* [[Griffitts:Gel recipes|Gel recipes]]
* [[Griffitts:Gel recipes|Gel recipes]]
* [[Griffitts:Common buffers|Sample buffer]]
* [[Griffitts:Common buffers|Sample buffer]]
* [[Griffitts:Bacterial lysis]]
* [[Griffitts:Bacterial lysis|Bacterial lysis]]
* [[Griffitts:6His purification]]
* [[Griffitts:6His purification|6His purification]]
* [[Griffitts:MBP purification]]
* [[Griffitts:MBP purification|MBP purification]]


==Bacteria==
==Bacteria==
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
* [[Griffitts:Common media#Bacterial Media and Additives|Media]]
* [[Griffitts:Stock_solutions#Antibiotics|Antibiotics]]
* [[Griffitts:Stock solutions#Antibiotics|Antibiotics]]
* [[Griffitts:Culture tips|Tips on streaking/culturing]]
* [[Griffitts:Culture tips|Tips on streaking/culturing]]
* [[Griffitts:Freezing bacteria|Freezing procedure]]
* [[Griffitts:Culture tips#Freeze Down|Freezing procedure]]
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep]]
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]]
* [[Griffitts:Bacterial RNA preparation|RNA prep (Trizol)]]
* [[Griffitts:Trizol purification|RNA prep (Trizol)]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]]
* [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]]
* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Triparental Mating|Triparental Mating]]
* [[Griffitts:Transduction|Transduction]]
* [[Griffitts:Transduction|Transduction]]
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]]
* [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]]
** [[Griffitts:Chemocompetent Cells#Transformation|Transformation]]
* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]]
* [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]]
** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]]
* [[Griffitts:LPS Analysis|LPS Analysis]]
* [[Griffitts:Competition|Competition Experiments]]
* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]]


==Plants==
==Plants==
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* [[Griffitts:Seed Storage|Seed Storage]]
* [[Griffitts:Seed Storage|Seed Storage]]
* [[Griffitts:Microscopy|Microscopy]]
* [[Griffitts:Microscopy|Microscopy]]
* [[Griffitts:Nodule Recovery|Nodule Bacteria Recovery]]
* [[Griffitts:Nodule Fixation|Nodule Fixation/Embedding]]
* [[Griffitts:Nodule Staining|LacZ Staining of Nodules]]

Latest revision as of 08:23, 19 April 2013