Griffitts:Protocols: Difference between revisions
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==DNA== | ==DNA== | ||
* [[Griffitts:Phylogenetics|Phylogenetics]] | |||
===PCR=== | ===PCR=== | ||
* [[Griffitts:PCR primers|PCR primers]] | * [[Griffitts:PCR primers|PCR primers]] | ||
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* [[Griffitts:Miniprep|Qiagen Miniprep]] | * [[Griffitts:Miniprep|Qiagen Miniprep]] | ||
* [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | * [[Griffitts:Low-copy plasmid prep|Low-copy plasmid prep]] | ||
* [[Griffitts:Midiprep|Nucleobond Midiprep]] | |||
* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | |||
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | * [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | ||
* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | |||
* [[Griffitts:Restriction digest|Restriction digest]] | * [[Griffitts:Restriction digest|Restriction digest]] | ||
* [[Griffitts:In-gel ligation|In-gel ligation]] | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
| Line 42: | Line 47: | ||
* [[Griffitts:Trizol purification|RNA prep (Trizol)]] | * [[Griffitts:Trizol purification|RNA prep (Trizol)]] | ||
* [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | * [[Griffitts:ß-Gal/GUS assay|ß-Gal/GUS assay]] | ||
* [[Griffitts:Bacterial Staining|LIVE/DEAD BacLight Staining]] | |||
* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* [[Griffitts:Transduction|Transduction]] | * [[Griffitts:Transduction|Transduction]] | ||
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** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ||
* [[Griffitts:LPS Analysis|LPS Analysis]] | * [[Griffitts:LPS Analysis|LPS Analysis]] | ||
* [[Griffitts:Competition|Competition Experiments]] | |||
* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | |||
==Plants== | ==Plants== | ||
Latest revision as of 08:23, 19 April 2013
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
- Competition Experiments
- Modified Eckhardt gel
