Griffitts:Protocols: Difference between revisions
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==DNA== | ==DNA== | ||
*[[Griffitts:Miniprep|Qiagen Miniprep]] | *[[Griffitts:Miniprep|Qiagen Miniprep]] | ||
*Taq PCR | *[[Griffitts:TaqPCR|Taq PCR]] | ||
*Pfx PCR | *Pfx PCR | ||
*Overlap-extension PCR | *Overlap-extension PCR | ||
Revision as of 09:37, 15 August 2007
DNA
- Qiagen Miniprep
- Taq PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- Restriction Digest
- Low-melt Gel Purification
- In-gel Ligation
RNA
- Trizol(Bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Freezing Procedure
- DNA prep
- RNA prep
- Triparental Mating
- Transduction
- Tips on streaking
Plants
- Seed scarification
- Media
- DNA prep
- RNA prep
- Seed Storage
- Microscopy
