Griffitts:Protocols: Difference between revisions
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* [[Griffitts:Triparental Mating|Triparental Mating]] | * [[Griffitts:Triparental Mating|Triparental Mating]] | ||
* [[Griffitts:Transduction|Transduction]] | * [[Griffitts:Transduction|Transduction]] | ||
* [[Griffitts: | * [[Griffitts:Chemocompetent Cells|Chemocompetent Cells]] | ||
* [[Griffitts: | * [[Griffitts:Electrocompetent Cells|Electrocompetent Cells]] | ||
==Plants== | ==Plants== | ||
Revision as of 14:18, 28 January 2008
Recipes
DNA
PCR
- PCR primers
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Sequencing prep
DNA prep
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
