Griffitts:Protocols: Difference between revisions
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* [[Griffitts:Gel recipes|Gel recipes]] | * [[Griffitts:Gel recipes|Gel recipes]] | ||
* [[Griffitts:Common buffers|Sample buffer]] | * [[Griffitts:Common buffers|Sample buffer]] | ||
* [[Griffitts:Bacterial lysis]] | * [[Griffitts:Bacterial lysis|Bacterial lysis]] | ||
* [[Griffitts:6His purification]] | * [[Griffitts:6His purification|6His purification]] | ||
* [[Griffitts:MBP purification]] | * [[Griffitts:MBP purification|MBP purification]] | ||
==Bacteria== | ==Bacteria== | ||
Revision as of 12:09, 13 February 2008
Recipes
DNA
PCR
- PCR primers
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Sequencing prep
DNA prep
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
