Griffitts:Protocols: Difference between revisions
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** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ** [[Griffitts:Electrocompetent Cells#Transformation|Transformation]] | ||
* [[Griffitts:LPS Analysis|LPS Analysis]] | * [[Griffitts:LPS Analysis|LPS Analysis]] | ||
* [[Griffitts:Competition|Competition Experiments]] | |||
==Plants== | ==Plants== | ||
Revision as of 13:45, 20 February 2013
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Nucleobond Midiprep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- LIVE/DEAD BacLight Staining
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
- Competition Experiments
