Griffitts:Protocols: Difference between revisions
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*MBP purification (MBP) | *MBP purification (MBP) | ||
==Bacteria== | ==Bacteria== | ||
*Media | *[[Griffitts:Common media#Bacterial Media and Additives|Media]] | ||
*Antibiotics | *Antibiotics | ||
*Tips on streaking/culturing | *Tips on streaking/culturing | ||
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*Triparental Mating | *Triparental Mating | ||
*Transduction | *Transduction | ||
==Plants== | ==Plants== | ||
*Media | *Media | ||
Revision as of 12:07, 8 September 2007
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Taq PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing Procedure
- Genomic DNA prep
- RNA prep (Trizol)
- Beta-gal/gus assays
- Triparental Mating
- Transduction
Plants
- Media
- Seed scarification
- Tips for growth/seed storage
- DNA prep
- RNA prep
- Seed Storage
- Microscopy
