Griffitts:Protocols: Difference between revisions
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* [[Griffitts:Common buffers|Common buffers]] | * [[Griffitts:Common buffers|Common buffers]] | ||
* [[Griffitts:Common media|Common media]] | * [[Griffitts:Common media|Common media]] | ||
* [[ | * [[Griffitts:Stock solutions|Stock solutions]] | ||
==DNA== | ==DNA== | ||
Revision as of 13:05, 6 November 2007
Recipes
DNA
- Qiagen Miniprep
- Low-copy plasmid prep
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Gel electrophoresis
- Restriction digest
- In-gel ligation
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Bacterial lysis
- 6His purification (6His)
- MBP purification (MBP)
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Competent Cells
- Electroporation
