Griffitts:Protocols: Difference between revisions
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m (→Bacteria) |
m (→Protein) |
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| Line 27: | Line 27: | ||
*5'RACE | *5'RACE | ||
==Protein== | ==Protein== | ||
*[[Griffitts:Gel recipes|Gel recipes]] | * [[Griffitts:Gel recipes|Gel recipes]] | ||
*Sample buffer | * [[Griffitts:Common buffers|Sample buffer]] | ||
*Bacterial lysis | * [[Griffitts:Bacterial lysis]] | ||
*6His purification | * [[Griffitts:6His purification]] | ||
*MBP purification | * [[Griffitts:MBP purification]] | ||
==Bacteria== | ==Bacteria== | ||
Revision as of 12:09, 13 February 2008
Recipes
DNA
PCR
- PCR primers
- Standard PCR
- Pfx PCR
- Overlap-extension PCR
- Arbitrary PCR
- PCR clean-up (Qiagen)
- Sequencing prep
DNA prep
RNA
- Trizol purification (bacterial)
- DNAse treatment
- Reverse transcription
- 5'RACE
Protein
- Gel recipes
- Sample buffer
- Griffitts:Bacterial lysis
- Griffitts:6His purification
- Griffitts:MBP purification
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
