Griffitts:Protocols: Difference between revisions
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* [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | * [[Griffitts:Bacterial DNA preparation|Genomic DNA prep (Qiagen)]] | ||
* [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | * [[Griffitts:Gel electrophoresis|Gel electrophoresis]] | ||
* [[Griffitts:Modified Eckhardt gel|Modified Eckhardt gel]] | |||
* [[Griffitts:Restriction digest|Restriction digest]] | * [[Griffitts:Restriction digest|Restriction digest]] | ||
* [[Griffitts:In-gel ligation|In-gel ligation]] | * [[Griffitts:In-gel ligation|In-gel ligation]] | ||
Revision as of 12:45, 5 March 2010
Recipes
DNA
PCR
DNA prep
- Qiagen Miniprep
- Low-copy plasmid prep
- Genomic DNA prep (Qiagen)
- Gel electrophoresis
- Modified Eckhardt gel
- Restriction digest
- In-gel ligation
RNA
Protein
Bacteria
- Media
- Antibiotics
- Tips on streaking/culturing
- Freezing procedure
- Genomic DNA prep (Qiagen)
- RNA prep (Trizol)
- ß-Gal/GUS assay
- Triparental Mating
- Transduction
- Chemocompetent Cells
- Electrocompetent Cells
- LPS Analysis
