Griffitts:Protocols

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DNA

• Qiagen Miniprep
• Taq PCR
• Pfx PCR
• Overlap-extension PCR
• Arbitrary PCR
• Restriction Digest
• Low-melt Gel Purification
• In-gel Ligation

RNA

• Trizol(Bacterial)
• DNAse treatment
• Reverse transcription
• 5'RACE

Protein

• Gel recipes
• Sample buffer
• Bacterial lysis
• 6His purification (6His)
• MBP purification (MBP)

Bacteria

• Media
• Freezing Procedure
• DNA prep
• RNA prep
• Triparental Mating
• Transduction
• Tips on streaking

Plants

• Seed scarification
• Media
• DNA prep
• RNA prep
• Seed Storage
• Microscopy