Griffitts:Protocols

From OpenWetWare

Revision as of 12:37, 15 August 2007 by Joel S Griffitts (Talk | contribs)
Jump to: navigation, search

Home     Research     Lab Members     Protocols     Publications     Internal    


Contents

DNA

  • Qiagen Miniprep
  • Taq PCR
  • Pfx PCR
  • Overlap-extension PCR
  • Arbitrary PCR
  • Restriction Digest
  • Low-melt Gel Purification
  • In-gel Ligation

RNA

  • Trizol(Bacterial)
  • DNAse treatment
  • Reverse transcription
  • 5'RACE

Protein

  • Gel recipes
  • Sample buffer
  • Bacterial lysis
  • 6His purification (6His)
  • MBP purification (MBP)

Bacteria

  • Media
  • Freezing Procedure
  • DNA prep
  • RNA prep
  • Triparental Mating
  • Transduction
  • Tips on streaking

Plants

  • Seed scarification
  • Media
  • DNA prep
  • RNA prep
  • Seed Storage
  • Microscopy
Personal tools