Griffitts:Protocols

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Home Research Lab Members Protocols Publications Internal Emeritus

Recipes

DNA

Qiagen Miniprep
Low-copy plasmid prep
Standard PCR
Pfx PCR
Overlap-extension PCR
Arbitrary PCR
Restriction digest
In-gel ligation

RNA

Trizol purification (bacterial)
DNAse treatment
Reverse transcription
5'RACE

Protein

Gel recipes
Sample buffer
Bacterial lysis
6His purification (6His)
MBP purification (MBP)

Bacteria

Media
Antibiotics
Tips on streaking/culturing
Freezing Procedure
Genomic DNA prep
RNA prep (Trizol)
ß-Gal/GUS assay
Triparental Mating
Transduction

Plants

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