Griffitts:Stock solutions: Difference between revisions
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* 1 g [[Wikipedia:Ampicillin|Ampicillin]] | * 1 g [[Wikipedia:Ampicillin|Ampicillin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter | * Filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Chloramphenicol (40 mL)=== | ===Chloramphenicol (40 mL)=== | ||
* 0.3 g [[Wikipedia:Chloramphenicol|Chloramphenicol]] | * 0.3 g [[Wikipedia:Chloramphenicol|Chloramphenicol]] | ||
* 40 mL [[Wikipedia:Ethanol|Ethanol]] | * 40 mL [[Wikipedia:Ethanol|Ethanol]] | ||
** Do '''not''' filter | ** Do '''not''' filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Gentamycin (40 mL)=== | ===Gentamycin (40 mL)=== | ||
* 0.3 g [[Wikipedia:Gentamycin|Gentamycin]] | * 0.3 g [[Wikipedia:Gentamycin|Gentamycin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter | * Filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Hygromycin (40 mL)=== | ===Hygromycin (40 mL)=== | ||
| Line 20: | Line 20: | ||
* 0.1 g [[Wikipedia:Kanamycin|Kanamycin]] | * 0.1 g [[Wikipedia:Kanamycin|Kanamycin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter | * Filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Neomycin (40 mL)=== | ===Neomycin (40 mL)=== | ||
* 0.8 g [[Wikipedia:Neomycin|Neomycin]] | * 0.8 g [[Wikipedia:Neomycin|Neomycin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter | * Filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Spectinomycin (40 mL)=== | ===Spectinomycin (40 mL)=== | ||
* 20 mg [[Wikipedia:Spectinomycin|Spectinomycin]] | * 20 mg [[Wikipedia:Spectinomycin|Spectinomycin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter | * Filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Streptomycin (40 mL)=== | ===Streptomycin (40 mL)=== | ||
* 2.0 g [[Wikipedia:Streptomycin|Streptomycin]] | * 2.0 g [[Wikipedia:Streptomycin|Streptomycin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter | * Filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
===Tetracycline (40 mL)=== | ===Tetracycline (40 mL)=== | ||
* 0.1 g [[Wikipedia:Tetracycline|Tetracycline]] | * 0.1 g [[Wikipedia:Tetracycline|Tetracycline]] | ||
* 40 mL [[Wikipedia:Ethanol|Ethanol]] | * 40 mL [[Wikipedia:Ethanol|Ethanol]] | ||
** Do '''not''' filter | ** Do '''not''' filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
==DNA== | ==DNA== | ||
===10 mM dNTPs=== | ===10 mM dNTPs=== | ||
Revision as of 14:24, 1 November 2007
Antibiotics
Ampicillin (40 mL)
- 1 g Ampicillin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Chloramphenicol (40 mL)
- 0.3 g Chloramphenicol
- 40 mL Ethanol
- Do not filter sterilize
- Store in refrigerator
Gentamycin (40 mL)
- 0.3 g Gentamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Hygromycin (40 mL)
Kanamycin (40 mL)
- 0.1 g Kanamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Neomycin (40 mL)
- 0.8 g Neomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Spectinomycin (40 mL)
- 20 mg Spectinomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Streptomycin (40 mL)
- 2.0 g Streptomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Tetracycline (40 mL)
- 0.1 g Tetracycline
- 40 mL Ethanol
- Do not filter sterilize
- Store in refrigerator
DNA
10 mM dNTPs
- Thaw and mix 100 mM stock solutions of G, A, T, C (NEB)
- Combine in a 1.5-mL tube:
- 60 μl H2O
- 10 μl G
- 10 μl A
- 10 μl T
- 10 μl C
