Griffitts:Stock solutions: Difference between revisions
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===20X Triton X-100=== | ===20X Triton X-100=== | ||
==Media Additives== | ==Media Additives== | ||
===Calcium Chloride (75 mL)=== | ===1 M Calcium Chloride (75 mL)=== | ||
* 75 mL dH<sub>2</sub>O | * 75 mL dH<sub>2</sub>O | ||
* 11.027 g CaCl<sub>2</sub>·2H<sub>2</sub>O | * 11.027 g CaCl<sub>2</sub>·2H<sub>2</sub>O | ||
Autoclave | Autoclave | ||
===X-gal=== | ===X-gal=== | ||
===IPTG=== | ===IPTG=== | ||
===Copper sulfate=== | ===Copper sulfate=== | ||
===SDS=== | ===SDS=== | ||
Revision as of 11:13, 14 January 2008
Antibiotics
Ampicillin (40 mL)
- 1 g Ampicillin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Chloramphenicol (40 mL)
- 0.3 g Chloramphenicol
- 40 mL Ethanol
- Do not filter sterilize
- Store in refrigerator
Gentamycin (40 mL)
- 0.3 g Gentamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Hygromycin (40 mL)
Kanamycin (40 mL)
- 0.1 g Kanamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Neomycin (40 mL)
- 0.8 g Neomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Spectinomycin (40 mL)
- 20 mg Spectinomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Streptomycin (40 mL)
- 2.0 g Streptomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Tetracycline (40 mL)
- 0.1 g Tetracycline
- 40 mL Ethanol
- Do not filter sterilize
- Store in refrigerator
DNA
10 mM dNTPs
- Thaw and mix 100 mM stock solutions of G, A, T, C (NEB)
- Combine in a 1.5-mL tube:
10X Taq buffer
10X Pfx buffer
20X Triton X-100
Media Additives
1 M Calcium Chloride (75 mL)
- 75 mL dH2O
- 11.027 g CaCl2·2H2O
Autoclave
