Griffitts:Stock solutions: Difference between revisions
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===Chloramphenicol (40 mL)=== | ===Chloramphenicol (40 mL)=== | ||
* 0.3 g [[Wikipedia:Chloramphenicol|Chloramphenicol]] | * 0.3 g [[Wikipedia:Chloramphenicol|Chloramphenicol]] | ||
* 40 mL [[Wikipedia:Ethanol|Ethanol]] | * 40 mL 50% [[Wikipedia:Ethanol|Ethanol]] | ||
** Do '''not''' filter sterilize | ** Do '''not''' filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
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** Store in refrigerator | ** Store in refrigerator | ||
===Spectinomycin (40 mL)=== | ===Spectinomycin (40 mL)=== | ||
* | * 0.8 g [[Wikipedia:Spectinomycin|Spectinomycin]] | ||
* 40 mL ddH<sub>2</sub>O | * 40 mL ddH<sub>2</sub>O | ||
* Filter sterilize | * Filter sterilize | ||
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===Tetracycline (40 mL)=== | ===Tetracycline (40 mL)=== | ||
* 0.1 g [[Wikipedia:Tetracycline|Tetracycline]] | * 0.1 g [[Wikipedia:Tetracycline|Tetracycline]] | ||
* 40 mL [[Wikipedia:Ethanol|Ethanol]] | * 40 mL 50% [[Wikipedia:Ethanol|Ethanol]] | ||
** Do '''not''' filter sterilize | ** Do '''not''' filter sterilize | ||
** Store in refrigerator | ** Store in refrigerator | ||
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! style="background:#9400D3;" | <font color=white>'''PURPLE'''</font> | ! style="background:#9400D3;" | <font color=white>'''PURPLE'''</font> | ||
| 7.5 mg/mL | | 7.5 mg/mL | ||
| 3 μg/mL ( | | 3 μg/mL (2500X) | ||
| 15 μg/mL (500X) | | 15 μg/mL (500X) | ||
|- | |- |
Revision as of 15:21, 4 February 2008
Antibiotics
Ampicillin (40 mL)
- 1 g Ampicillin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Chloramphenicol (40 mL)
- 0.3 g Chloramphenicol
- 40 mL 50% Ethanol
- Do not filter sterilize
- Store in refrigerator
Gentamycin (40 mL)
- 0.3 g Gentamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Hygromycin (40 mL)
Kanamycin (40 mL)
- 0.1 g Kanamycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Neomycin (40 mL)
- 0.8 g Neomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Spectinomycin (40 mL)
- 0.8 g Spectinomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Streptomycin (40 mL)
- 2.0 g Streptomycin
- 40 mL ddH2O
- Filter sterilize
- Store in refrigerator
Tetracycline (40 mL)
- 0.1 g Tetracycline
- 40 mL 50% Ethanol
- Do not filter sterilize
- Store in refrigerator
Antibiotic | Abbr. | Stripe | Stock | E. coli | S. meliloti |
---|---|---|---|---|---|
Ampicillin | Ap | BLACK | 25 mg/mL | 100 μg/mL (250X) | N/A |
Chloramphenicol | Cm | BROWN | 7.5 mg/mL | 30 μg/mL (250X) | N/A |
Gentamycin | Gm | PURPLE | 7.5 mg/mL | 3 μg/mL (2500X) | 15 μg/mL (500X) |
Hygromycin | Hy | (none) | 5 mg/mL | 20 μg/mL (250X) | 20 μg/mL (250X) |
Kanamycin | Km | YELLOW | 7.5 mg/mL | 30 μg/mL (250X) | N/A |
Neomycin | Nm | GREEN | 20 mg/mL | N/A | 80 μg/mL (250X) |
Spectinomycin | Sp | ORANGE | 20 mg/mL | 80 μg/mL (250X) | 80 μg/mL (250X) |
Streptomycin | Sm | BLUE | 50 mg/mL | N/A | 200 μg/mL (250X) |
Tetracycline | Tc | RED | 2.5 mg/mL | 10 μg/mL (250X) | 10 μg/mL (250X) |
Note: These parameters are intended to be compatible with pre-made 250-mL amounts of LB agar, such that usually 1 mL of stock solution is diluted into the LB agar. For liquid LB (typically 75 mL amounts) 0.3 mL of the stock solution will usually be added. Exception: Gentamycin for E. coli.
DNA
10 mM dNTPs
- Thaw and mix (stir) 100 mM stock solutions of G, A, T, C (NEB)
- Combine in a 1.5-mL tube:
10X Taq buffer
10X Pfx buffer
20X Triton X-100
Media Additives
1 M Calcium Chloride (75 mL)
- 75 mL dH2O
- 11.027 g CaCl2·2H2O
Autoclave