Griffitts:Stock solutions - Revision history

Matthew B. Crook: /* Gentamicin (40 mL) */ changed to 20 mL

2013-03-19T16:36:09Z

Gentamicin (40 mL): changed to 20 mL

←Older revision		Revision as of 16:36, 19 March 2013
Line 21:		Line 21:
	* Do '''not''' filter sterilize—the ethanol may dissolve the filter		* Do '''not''' filter sterilize—the ethanol may dissolve the filter
	NOTE: Store at –20°C		NOTE: Store at –20°C
-	===Gentamicin (40 mL)===	+	===Gentamicin (20 mL)===
	For a stock solution of 7.5 mg/mL:		For a stock solution of 7.5 mg/mL:
-	* ~~300~~ mg [[Wikipedia:Gentamicin\|Gentamicin]]	+	* 150 mg [[Wikipedia:Gentamicin\|Gentamicin]]
-	* 40 mL ddH<sub>2</sub>O	+	* 20 mL ddH<sub>2</sub>O
	* Filter sterilize		* Filter sterilize
	NOTE: Store at 4°C		NOTE: Store at 4°C
		+
	===Hygromycin (40 mL)===		===Hygromycin (40 mL)===
	For a stock solution of 5 mg/mL:		For a stock solution of 5 mg/mL:

Matthew B. Crook: /* Triclosan (40 mL) */

2012-02-29T22:41:34Z

Triclosan (40 mL)

←Older revision		Revision as of 22:41, 29 February 2012
Line 85:		Line 85:
	===Triclosan (40 mL)===		===Triclosan (40 mL)===
	For a stock solution of 10 mg/mL:		For a stock solution of 10 mg/mL:
-	* 400 mg [[Wikipedia:~~Tetracycline~~\|~~Tetracycline~~]]	+	* 400 mg [[Wikipedia:Triclosan\|Triclosan]]
	* 40 mL 100% [[Wikipedia:Ethanol\|Ethanol]]		* 40 mL 100% [[Wikipedia:Ethanol\|Ethanol]]
	NOTE: Do '''not''' filter sterilize—the ethanol may dissolve the filter<br>		NOTE: Do '''not''' filter sterilize—the ethanol may dissolve the filter<br>
	NOTE: Store at 4°C		NOTE: Store at 4°C
		+
	===Trimethoprim (40 mL)===		===Trimethoprim (40 mL)===
	For a stock solution of 6.25 mg/mL:		For a stock solution of 6.25 mg/mL:

Matthew B. Crook: /* 1 M Sodium Citrate (40 mL) */

2012-02-11T21:14:38Z

1 M Sodium Citrate (40 mL)

←Older revision		Revision as of 21:14, 11 February 2012
Line 303:		Line 303:

	===1 M Sodium Citrate (40 mL)===		===1 M Sodium Citrate (40 mL)===
-	* 40 mL ddH<sub>2</sub>O	+	* 35 mL ddH<sub>2</sub>O
	* 11.76 g sodium citrate tribasic dihydrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O)		* 11.76 g sodium citrate tribasic dihydrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O)
-	* pH to 5.7 with HCl	+	* pH to 5.7 with HCl (~1 mL)
		+	* QS to 40 mL with ddH<sub>2</sub>O
	Sterile filter		Sterile filter

Matthew B. Crook: /* DNA ladder */

2011-05-16T16:55:45Z

DNA ladder

←Older revision		Revision as of 16:55, 16 May 2011
Line 272:		Line 272:
	* 1/50 dilution of [[Griffitts:Common buffers#2% bromophenol blue\|2% bromophenol blue]]		* 1/50 dilution of [[Griffitts:Common buffers#2% bromophenol blue\|2% bromophenol blue]]
	===DNA ladder===		===DNA ladder===
-	* ~~147~~ μL ddH<sub>2</sub>0	+	* 140 μL ddH<sub>2</sub>0
-	* 34 μL [[Griffitts:Common buffers#8X DNA loading\|8X DNA loading]] buffer	+	* 40 μL [[Griffitts:Common buffers#8X DNA loading\|8X DNA loading]] buffer
	* 20 μL 1 kb ladder from NEB		* 20 μL 1 kb ladder from NEB
	* 2 μL [[Griffitts:Common buffers#0.25 M EDTA (75 mL)\|0.25 M EDTA]]<br>		* 2 μL [[Griffitts:Common buffers#0.25 M EDTA (75 mL)\|0.25 M EDTA]]<br>
	Note: use 5 μL per lane; 3kb band = 60 ng		Note: use 5 μL per lane; 3kb band = 60 ng
		+
	===Ethidium Bromide (1 mL)===		===Ethidium Bromide (1 mL)===
	* 100 μL 10 mg/mL ethidium bromide		* 100 μL 10 mg/mL ethidium bromide

Matthew B. Crook at 20:50, 11 April 2011

2011-04-11T20:50:19Z

←Older revision		Revision as of 20:50, 11 April 2011
Line 40:		Line 40:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Nalidixic acid (30 mL)===		===Nalidixic acid (30 mL)===
-	For a stock solution of 5 mg/mL:	+	For a stock solution of 30 mg/mL:
	* 900 mg [[Wikipedia:Nalidixic Acid\|Nalidixic Acid]]		* 900 mg [[Wikipedia:Nalidixic Acid\|Nalidixic Acid]]
	* 360 mg NaOH		* 360 mg NaOH
Line 166:		Line 166:
	\| Na		\| Na
	\| <center>(none)</center>		\| <center>(none)</center>
-	\| 5 mg/mL	+	\| 30 mg/mL
	\| 30 μg/mL (1000X)		\| 30 μg/mL (1000X)
	\| N/A		\| N/A

Matthew B. Crook: /* Streptomycin (40 mL) */

2011-03-07T22:17:58Z

Streptomycin (40 mL)

←Older revision		Revision as of 22:17, 7 March 2011
Line 71:		Line 71:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Streptomycin (40 mL)===		===Streptomycin (40 mL)===
-	For a stock solution of 25 mg/mL:	+	For a stock solution of 50 mg/mL:
	* 2 g [[Wikipedia:Streptomycin\|Streptomycin]]		* 2 g [[Wikipedia:Streptomycin\|Streptomycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
	* Filter sterilize		* Filter sterilize
	NOTE: Store at 4°C		NOTE: Store at 4°C
		+
	===Tetracycline (40 mL)===		===Tetracycline (40 mL)===
	For a stock solution of 2.5 mg/mL:		For a stock solution of 2.5 mg/mL:

Matthew B. Crook: /* Antibiotics Table */

2011-01-19T17:30:46Z

Antibiotics Table

←Older revision		Revision as of 17:30, 19 January 2011
Line 106:		Line 106:
	! width="120" style="background:#efefef;" \| ''S. meliloti''		! width="120" style="background:#efefef;" \| ''S. meliloti''
	! width="90" style="background:#efefef;" \| Solvent		! width="90" style="background:#efefef;" \| Solvent
-	! width="60" style="background:#efefef;" \| Storage	+	! width="90" style="background:#efefef;" \| Storage
	\|-		\|-
	\| Ampicillin		\| Ampicillin

Matthew B. Crook: /* Antibiotics Table */

2011-01-19T17:30:22Z

Antibiotics Table

←Older revision		Revision as of 17:30, 19 January 2011
Line 220:		Line 220:
	! style="background:#FF0000;" \| <font color=white>'''RED'''</font>		! style="background:#FF0000;" \| <font color=white>'''RED'''</font>
	\| 2.5 mg/mL		\| 2.5 mg/mL
-	\| 10 μg/mL (~~250X~~)	+	\| 5 μg/mL (500X)
-	\| 10 μg/mL (~~250X~~)	+	\| 5 μg/mL (500X)
	\| 50% EtOH		\| 50% EtOH
	\| 4°C		\| 4°C

Matthew B. Crook: /* Antibiotics */ Added stock concentrations to recipes

2010-10-04T19:00:00Z

Antibiotics: Added stock concentrations to recipes

←Older revision		Revision as of 19:00, 4 October 2010
Line 4:		Line 4:
	==Antibiotics==		==Antibiotics==
	===Ampicillin (40 mL)===		===Ampicillin (40 mL)===
		+	For a stock solution of 25 mg/mL:
	* 1 g [[Wikipedia:Ampicillin\|Ampicillin]]		* 1 g [[Wikipedia:Ampicillin\|Ampicillin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 9:		Line 10:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Chloramphenicol (40 mL)===		===Chloramphenicol (40 mL)===
		+	For a stock solution of 7.5 mg/mL:
	* 300 mg [[Wikipedia:Chloramphenicol\|Chloramphenicol]]		* 300 mg [[Wikipedia:Chloramphenicol\|Chloramphenicol]]
	* 40 mL 50% [[Wikipedia:Ethanol\|Ethanol]]		* 40 mL 50% [[Wikipedia:Ethanol\|Ethanol]]
Line 14:		Line 16:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Erythromycin (20 mL)===		===Erythromycin (20 mL)===
		+	For a stock solution of 10 mg/mL:
	* 200 mg [[Wikipedia:Erythromycin\|Erythromycin]]		* 200 mg [[Wikipedia:Erythromycin\|Erythromycin]]
	* 20 mL 100% ethanol (in fume hood)		* 20 mL 100% ethanol (in fume hood)
Line 19:		Line 22:
	NOTE: Store at –20°C		NOTE: Store at –20°C
	===Gentamicin (40 mL)===		===Gentamicin (40 mL)===
		+	For a stock solution of 7.5 mg/mL:
	* 300 mg [[Wikipedia:Gentamicin\|Gentamicin]]		* 300 mg [[Wikipedia:Gentamicin\|Gentamicin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 24:		Line 28:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Hygromycin (40 mL)===		===Hygromycin (40 mL)===
		+	For a stock solution of 5 mg/mL:
	* 200 mg [[Wikipedia:Hygromycin\|Hygromycin]]		* 200 mg [[Wikipedia:Hygromycin\|Hygromycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 29:		Line 34:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Kanamycin (40 mL)===		===Kanamycin (40 mL)===
		+	For a stock solution of 7.5 mg/mL:
	* 300 mg [[Wikipedia:Kanamycin\|Kanamycin]]		* 300 mg [[Wikipedia:Kanamycin\|Kanamycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 34:		Line 40:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Nalidixic acid (30 mL)===		===Nalidixic acid (30 mL)===
		+	For a stock solution of 5 mg/mL:
	* 900 mg [[Wikipedia:Nalidixic Acid\|Nalidixic Acid]]		* 900 mg [[Wikipedia:Nalidixic Acid\|Nalidixic Acid]]
	* 360 mg NaOH		* 360 mg NaOH
Line 40:		Line 47:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Neomycin (40 mL)===		===Neomycin (40 mL)===
		+	For a stock solution of 25 mg/mL:
	* 1 g [[Wikipedia:Neomycin\|Neomycin]]		* 1 g [[Wikipedia:Neomycin\|Neomycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 45:		Line 53:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Phosphomycin===		===Phosphomycin===
		+	For a stock solution of 25 mg/mL:
	* 1 g [[Wikipedia:Fosfomycin\|Phosphomycin]]		* 1 g [[Wikipedia:Fosfomycin\|Phosphomycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 50:		Line 59:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Rifampicin (20 mL)===		===Rifampicin (20 mL)===
		+	For a stock solution of 50 mg/mL:
	* 1 g [[Wikipedia:Rifampicin\|Rifampicin]]		* 1 g [[Wikipedia:Rifampicin\|Rifampicin]]
	* 20 mL N,N-dimethylformamide (DMF; in fume hood)		* 20 mL N,N-dimethylformamide (DMF; in fume hood)
Line 55:		Line 65:
	NOTE: Store at –20°C		NOTE: Store at –20°C
	===Spectinomycin (40 mL)===		===Spectinomycin (40 mL)===
		+	For a stock solution of 20 mg/mL:
	* 800 mg [[Wikipedia:Spectinomycin\|Spectinomycin]]		* 800 mg [[Wikipedia:Spectinomycin\|Spectinomycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 60:		Line 71:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Streptomycin (40 mL)===		===Streptomycin (40 mL)===
		+	For a stock solution of 25 mg/mL:
	* 2 g [[Wikipedia:Streptomycin\|Streptomycin]]		* 2 g [[Wikipedia:Streptomycin\|Streptomycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
Line 65:		Line 77:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Tetracycline (40 mL)===		===Tetracycline (40 mL)===
		+	For a stock solution of 2.5 mg/mL:
	* 100 mg [[Wikipedia:Tetracycline\|Tetracycline]]		* 100 mg [[Wikipedia:Tetracycline\|Tetracycline]]
	* 40 mL 50% [[Wikipedia:Ethanol\|Ethanol]]		* 40 mL 50% [[Wikipedia:Ethanol\|Ethanol]]
Line 70:		Line 83:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Triclosan (40 mL)===		===Triclosan (40 mL)===
		+	For a stock solution of 10 mg/mL:
	* 400 mg [[Wikipedia:Tetracycline\|Tetracycline]]		* 400 mg [[Wikipedia:Tetracycline\|Tetracycline]]
	* 40 mL 100% [[Wikipedia:Ethanol\|Ethanol]]		* 40 mL 100% [[Wikipedia:Ethanol\|Ethanol]]
Line 75:		Line 89:
	NOTE: Store at 4°C		NOTE: Store at 4°C
	===Trimethoprim (40 mL)===		===Trimethoprim (40 mL)===
		+	For a stock solution of 6.25 mg/mL:
	* 250 mg [[Wikipedia:Trimethoprim\|Trimethoprim]]		* 250 mg [[Wikipedia:Trimethoprim\|Trimethoprim]]
	* 40 mL 100% [[Wikipedia:Dimethyl sulfoxide\|DMSO]]		* 40 mL 100% [[Wikipedia:Dimethyl sulfoxide\|DMSO]]

Matthew B. Crook: Added antibiotics

2010-05-15T20:30:06Z

Added antibiotics

←Older revision		Revision as of 20:30, 15 May 2010
Line 41:		Line 41:
	===Neomycin (40 mL)===		===Neomycin (40 mL)===
	* 1 g [[Wikipedia:Neomycin\|Neomycin]]		* 1 g [[Wikipedia:Neomycin\|Neomycin]]
		+	* 40 mL ddH<sub>2</sub>O
		+	* Filter sterilize
		+	NOTE: Store at 4°C
		+	===Phosphomycin===
		+	* 1 g [[Wikipedia:Fosfomycin\|Phosphomycin]]
	* 40 mL ddH<sub>2</sub>O		* 40 mL ddH<sub>2</sub>O
	* Filter sterilize		* Filter sterilize
Line 68:		Line 73:
	* 40 mL 100% [[Wikipedia:Ethanol\|Ethanol]]		* 40 mL 100% [[Wikipedia:Ethanol\|Ethanol]]
	NOTE: Do '''not''' filter sterilize—the ethanol may dissolve the filter<br>		NOTE: Do '''not''' filter sterilize—the ethanol may dissolve the filter<br>
		+	NOTE: Store at 4°C
		+	===Trimethoprim (40 mL)===
		+	* 250 mg [[Wikipedia:Trimethoprim\|Trimethoprim]]
		+	* 40 mL 100% [[Wikipedia:Dimethyl sulfoxide\|DMSO]]
		+	NOTE: Do '''not''' filter sterilize—the DMSO may dissolve the filter<br>
		+	NOTE: Store at 4°C

	==Antibiotics Table==		==Antibiotics Table==
Line 79:		Line 90:
	! width="120" style="background:#efefef;" \| ''E. coli''		! width="120" style="background:#efefef;" \| ''E. coli''
	! width="120" style="background:#efefef;" \| ''S. meliloti''		! width="120" style="background:#efefef;" \| ''S. meliloti''
-	! width="60" style="background:#efefef;" \| Solvent	+	! width="90" style="background:#efefef;" \| Solvent
	! width="60" style="background:#efefef;" \| Storage		! width="60" style="background:#efefef;" \| Storage
	\|-		\|-
Line 132:		Line 143:
	\| 7.5 mg/mL		\| 7.5 mg/mL
	\| 30 μg/mL (250X)		\| 30 μg/mL (250X)
-	\| ~~N/A~~	+	\| '''Use Neomycin'''
	\| ddH<sub>2</sub>O		\| ddH<sub>2</sub>O
	\| 4°C		\| 4°C
Line 148:		Line 159:
	\| Nm		\| Nm
	! style="background:#008000;" \| <font color=white>'''GREEN'''</font>		! style="background:#008000;" \| <font color=white>'''GREEN'''</font>
		+	\| 25 mg/mL
		+	\| '''Use Kanamycin'''
		+	\| 100 μg/mL (250X)
		+	\| ddH<sub>2</sub>O
		+	\| 4°C
		+	\|-
		+	\| Phosphomycin
		+	\| Pm
		+	\| <center>(none)</center>
	\| 25 mg/mL		\| 25 mg/mL
	\| N/A		\| N/A
Line 191:		Line 211:
	\|-		\|-
	\| Triclosan		\| Triclosan
-	\|	+	\| Tr
	\| <center>(none)</center>		\| <center>(none)</center>
	\| 10 mg/mL		\| 10 mg/mL
Line 197:		Line 217:
	\| N/A		\| N/A
	\| 100% EtOH		\| 100% EtOH
		+	\| 4°C
		+	\|-
		+	\| Trimethoprim
		+	\| Tm
		+	\| <center>(none)</center>
		+	\| 6.25 mg/mL
		+	\| N/A
		+	\| 12.5 μg/mL (500X)<br><center>or</center>25 μg/mL (250X)
		+	\| 100% DMSO
	\| 4°C		\| 4°C
	\|}		\|}