Griffitts:Stock solutions

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Contents

Antibiotics

Ampicillin (40 mL)

  • 1 g Ampicillin
  • 40 mL ddH2O
  • Filter sterilize
    • Store in refrigerator

Chloramphenicol (40 mL)

Gentamycin (40 mL)

  • 0.3 g Gentamycin
  • 40 mL ddH2O
  • Filter sterilize
    • Store in refrigerator

Hygromycin (40 mL)

Kanamycin (40 mL)

  • 0.1 g Kanamycin
  • 40 mL ddH2O
  • Filter sterilize
    • Store in refrigerator

Neomycin (40 mL)

  • 0.8 g Neomycin
  • 40 mL ddH2O
  • Filter sterilize
    • Store in refrigerator

Spectinomycin (40 mL)

  • 20 mg Spectinomycin
  • 40 mL ddH2O
  • Filter sterilize
    • Store in refrigerator

Streptomycin (40 mL)

  • 2.0 g Streptomycin
  • 40 mL ddH2O
  • Filter sterilize
    • Store in refrigerator

Tetracycline (40 mL)

DNA

10 mM dNTPs

  • Thaw and mix (stir) 100 mM stock solutions of G, A, T, C (NEB)
  • Combine in a 1.5-mL tube:
    • 60 μl H2O
    • 10 μl G
    • 10 μl A
    • 10 μl T
    • 10 μl C

10X Taq buffer

10X Pfx buffer

20X Triton X-100

Media Additives

1 M Calcium Chloride (75 mL)

  • 75 mL dH2O
  • 11.027 g CaCl2·2H2O

Autoclave

X-gal

IPTG

Copper sulfate

SDS

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