GroseLab:Protocols: Difference between revisions
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==Recipes== | ==Recipes== | ||
* [[ | * [[Common buffers|Common buffers]] | ||
* [[ | * [[Common media|Common media]] | ||
* [[ | * [[Stock solutions|Stock solutions]] | ||
= | ==Basic protocols== | ||
==DNA== | ===DNA=== | ||
* [[ | * [[TAQ PCR]] | ||
* [[ | * [[Phusion PCR]] | ||
* [[ | * [[Cloning]] | ||
==Protein== | ===Protein=== | ||
* [[ | * [[Western Blot]] | ||
=Bacterial Protocols= | ==Bacterial Protocols== | ||
===DNA=== | |||
*[[DH5α Competent Cells]] | |||
*[[Electrocompetent cells]] | |||
*[[Linear transformation]] | |||
[[ | ===Protein=== | ||
*[[HIS tag purification]] | |||
*[[GST purification]] | |||
*[[Myc purification]] | |||
*[[FLAG purification]] | |||
[[ | ==Yeast Protocols== | ||
===DNA=== | |||
*[[Competent Cells and Transformation]] | |||
*[[Quick Genomic DNA prep]] | |||
*[[Yeast Plasmid Prep]] | |||
[[ | ===Protein=== | ||
*[[IP kinase assays]] | |||
[[ | *[[in vivo PASK phosphoryation assay]] | ||
*[[in vivo Ugp1 phosphorylation assay]] | |||
*[[his tag purification]] | |||
*[[GST purification]] | |||
*[[Myc purification]] | |||
*[[FLAG purification]] | |||
Revision as of 15:03, 12 March 2011
