GroseLab:Protocols

From OpenWetWare

GroseLab

Recipes

• Common buffers
• Common media
• Stock solutions

Basic protocols

DNA

• TAQ PCR
• Phusion PCR
• Cloning

Protein

• Western Blot

Bacterial Protocols

DNA

• DH5α Competent Cells
• Electrocompetent cells
• Linear transformation

Protein

• HIS tag purification
• GST purification
• Myc purification
• FLAG purification

Yeast Protocols

DNA

• Competent Cells and Transformation
• Quick Genomic DNA prep
• Yeast Plasmid Prep

Protein

• IP kinase assays
• in vivo PASK phosphoryation assay
• in vivo Ugp1 phosphorylation assay
• his tag purification
• GST purification
• Myc purification
• FLAG purification