Harmer Lab:Protocols:Direct PCR: Difference between revisions
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<h3><font style="color:#F8B603;">100mL GES Buffer</font></h3> | <h3><font style="color:#F8B603;">100mL GES Buffer</font></h3> | ||
#1M Glycine, pH9, 10mL | #1M Glycine, pH9, 10mL | ||
# | #2M NaCl, 2.5mL | ||
#0.5M EDTA, pH8 200μL | #0.5M EDTA, pH8 200μL | ||
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Revision as of 09:09, 25 February 2011
Room 2123 |
96-Well Format DNA Extraction for Direct PCRModified from Bellstedt et al. 2010 by Matt
100mL Grinding BufferTo prepare 100 mL of grinding buffer dissolve all components except Tween 20 in double-distilled sterile water. Stir at low speed to avoid excessive foaming. Once all components have dissolved, adjust pH to 9.6 using NaOH solution. Then add Tween 20. Autoclave and store at 4°C.
100mL GES Buffer
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