Harmer Lab:Protocols:Direct PCR
96-Well Format DNA Extraction for Direct PCR
Modified from Bellstedt et al. 2010 by Matt
100mL Grinding Buffer
To prepare 100 mL of grinding buffer dissolve all components except Tween 20 in double-distilled sterile water. Stir at low speed to avoid excessive foaming. Once all components have dissolved, adjust pH to 9.6 using NaOH solution. Then add Tween 20. Autoclave and store at 4°C.
100mL GES Buffer