Harmer Lab:Protocols:Y2H: Difference between revisions
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Revision as of 11:12, 30 March 2009
Room 2123 |
Yeast 2-HybridNon-Selective Plates (1 litre) 46.7g Min. SD Agar base 0.64g -L/-T DO Supplement Selective Plates (1 litre) 46.7g Min. SD Agar base 0.60g -H/-L/-T DO Supplement 1ml α-gal Things to Consider Rinse DO supplement off weigh boat into media Dissolve w/stirring for 15min, then autoclave 121°C, 15min
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