Harmer Lab:Protocols:Y2H

Room 2123 Department of Plant Biology 1002 Life Sciences, One Shields Ave. University of California Davis Davis, CA 95616 Contact: slharmer at ucdavis.edu

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Yeast 2-Hybrid

Non-Selective Plates (1 litre) 46.7g Min. SD Agar base 0.64g -L/-T DO Supplement Selective Plates (1 litre) 46.7g Min. SD Agar base 0.60g -H/-L/-T DO Supplement 1ml α-gal

Handy Hints Rinse DO supplement off weigh boat into media Dissolve w/stirring for 15min, then autoclave 121°C, 15min

• Matthew A. Jones 14:12, 30 March 2009 (EDT):