Hartings AU Photosynthesis Lab:Materials/Hemoglobin: Difference between revisions
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<u>K31C</u> | <u>K31C</u> | ||
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. | This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - K31C Asc Hb in pET-3d) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as K31 Asc Hb). | ||
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence | K31C Sequence formatted with amino acids]] | [[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence | K31C Sequence formatted with amino acids]] | ||
[[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence_text | K31C Sequence as text]] | [[Hartings_AU_Photosynthesis_Lab:Materials/Hemoglobin/K31C_Sequence_text | K31C Sequence as text]] |
Revision as of 08:36, 14 January 2013
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Hemoglobin Mutants
Wild Type
This DNA was the gift of Daniel Goldberg at WUSL. The original articles for Goldberg's work can be found here and here. The gene is housed in the pET-3d vector, which has ampicillin resistance and no His6 sequence. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - WT Asc Hb in pET-3d 0.46μg/μL) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as WT Asc Hb).
WT Sequence formatted with amino acids
K31C
This vector housed in the pET-3d plasmid was prepared via a PCR mutation. The plasmid can be found in aqueous solution in the -20 freezer in Beeghly 203B (in the Hemoglobin box labeled - K31C Asc Hb in pET-3d) or as transformed into BL21 (DE3) cells as a glycerol stock in the -80 freezer in Beeghly 303 (in the Hartings Lab Glycerol stocks box labeled as K31 Asc Hb).