Haynes:BioBrick Method short

Assembly of BioBrick Parts

By Karmella Haynes, 2012

If you are new to molecular biology, please refer to a general biology reference (college biology text book, Wikipedia, etc.) or click the "ref" link for more details on the terms in bold print.

A BioBrick is a piece of DNA that is flanked by a community-wide accepted set of restriction enzyme cut sites (ref), e.g., EcoRI (E), NotI (N), XbaI (X) upstream (left); SpeI, NotI, PstI downstream (right). The standard shown below is RFC23, or the "Silver Standard" developed by Pam Silver's lab at Harvard:

gaattc gcggccgc a tctaga [BioBrick part] actagt a gcggccgctgcag
  EcoRI   NotI       XbaI                   SpeI     NotI   PstI