Haynes:GOEnrichment: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 12: | Line 12: | ||
# In the "Target Set" field, paste a list of genes that you want to analyze. | # In the "Target Set" field, paste a list of genes that you want to analyze. | ||
# For the Background set, upload complete list of all gene symbols for your organism. Use your own or one of the following: | # For the Background set, upload complete list of all gene symbols for your organism. Use your own or one of the following: | ||
## Human genes - GOBg_Human_092014.txt | ## Human genes - [http://openwetware.org/images/6/6c/GOBg_Human_092014.txt GOBg_Human_092014.txt] | ||
## Mouse genes - | ## Mouse genes - | ||
Revision as of 15:49, 11 September 2014
GOrilla
Intro: These instructions will help you to use the Gene Ontology enRIchment anaLysis and visuaLizAtion tool (GOrilla) to search for enriched GO terms in a target list of genes compared to a background list of genes. The software searches for GO terms that are enriched in the target set compared to the background set using the standard Hyper Geometric statistics.
- Go to http://cbl-gorilla.cs.technion.ac.il/
- Set "Choose organism" to the relevant organism (e.g., Homo sapiens = human, Mus musculus = mouse)
- Set "Choose running mode" to "Two unranked lists of genes (target and background lists)"
- In the "Target Set" field, paste a list of genes that you want to analyze.
- For the Background set, upload complete list of all gene symbols for your organism. Use your own or one of the following:
- Human genes - GOBg_Human_092014.txt
- Mouse genes -