Haynes:LitReviewOct2014

<- Back to Publications

JOURNAL ASSIGNMENTS:

• ACS Synthetic Biology - Rene
• Cell - Meli'sa
• Frontiers in Microbiotechnology – David
• Journal of Biological Engineering - Meli'sa
• Journal of Cell Biology - Jonah
• Molecular Biology of the Cell - David
• Molecular and Cellular Biology - Rene
• Nature - Jonah
• Nature Biotechnology - Ryan
• Nature Methods - Jan
• Nature Molecular Systems Biology - Ryan
• Public Library of Science Biology (PLoS Biology) - Cameron
• Proceedings of the National Academy of Sciences - Jan
• Science - Cameron
• Miscellaneous Reviews and Media - Dr. Haynes

INSTRUCTIONS: Please search for lab-relevant articles dated May 9, 2014 up to today.

Spring 2014, 05/08/2014

Use the following text format EXACTLY as it is shown below...

1. (year) Title. Author One, Author Two, and Author Three et al. Journal. Volume:pages. Link.
   Summary: Very short explanation of why this paper is relevant/ interesting.
   
   
2. (2011) Engineering a Photoactivated Caspase-7 for Rapid Induction of Apoptosis. Evan Mills, Xi Chen, Elizabeth Pham, Stanley Wong, and Kevin Truong et al. ACS Synthetic Biology, 1.3:75-82. Link.
   Summary: A group from University of Toronto developed a protein that causes rapid apotosis (cell death) of targeted cells.
   
   

Open edit mode and copy the example above. Do not erase the <br><br> tags. Do not use keyboard line returns to space out the numbered list, or else each item will start with the number 1.

ACS Synthetic Biology

1. (2014) Conditional control of mammalian gene expression by tetracycline- dependent hammerhead ribozymes. Kim Beilstein, Alexander Wittmann, Manuel Grez, et al. ACS Synthetic Biology. Published online ahead of print. Link.
   Summary: The authors demonstrated the functionality of a 3'-UTR rybozyme that results in correct folding of the mRNA after addition of tet. Important because the mammalian synthetic biology toolbox is limited so more parts are valuable (even though this want can't really be used with the well-characterized TetR system), eliminates basal pTet expression, and it removes an integration step for building a transgenic inducible system.
   
   
2. (2014) Environmental Sensing of Heavy Metals Through Whole Cell Microbial Biosensors: A Synthetic Biology Approach Lara Bereza-Malcolm, Gulay Mann, and Ashley Edwin Franks. ACS Synthetic Biology, Published online ahead of print. Link.
   Summary: People use quorum sensing to build microbial biosensors. Good resource for QS review.
   
   

Cell

1. (2014) Ruled by Ubiquitylation: A New Order for Polycomb Recruitment. Yuri B Schwartz, Vincenzo Pirrotta et al. Cell, 8.2:321-325. Link
   Summary: This paper provides a new way of thinking about Polycomb complexes and how they play a role in gene regulation! Everything we currently know about polycomb complex recruitment is going to be challenged. The recruitment of polycomb complexes have always been studied in two steps. First, the PRC2 produces histone H3K27 trimethylation at a specific gene, then, the PRC1 complex is recruited by its ability to bind to H3k27me3. However, there have been articles that contradict this process by focusing on a variant of the PRC1 complex and H2A ubiquitylation of surrounding chromatin in the recruitment of PRC2 and H3k27.
   
   
2. (2014) Generation of Functional Human Pancreatic β Cells in Vitro. Felicia W. Paglicua, Jeffery R. Millman, Mads Gurtler et al. Cell, 159:2, 428-439. [1]
   Summary: Attn. David; This research focused on generating glucose responsive β cells from hPSC with limited genetic differences. This, of course, could play a very important role in diabetes treatment.

Frontiers in Microbiotechnology

None. Closest was an article on tools for designing synthetic genes.

1. (2014) Computational tools and algorithms for designing customized synthetic genes. Nathan Gould, Oliver Hendy, and Dimitris Papamichail. Frontiers in Bioengineering and Biotechnology. 2:41.Link. Summary: Large summary of software tools for the design of synthetic genes. Tools include DNAWorks, Jcat, Synthetic Gene Designer etc. Also examines criteria for optimizing gene expression in created synthetic genes.

Journal of Biological Engineering

No relevant articles.

Journal of Cell Biology

1. TBA

Molecular Biology of the Cell

1. TBA

Molecular and Cellular Biology

1. TBA

Nature

1. TBA

Nature Biotechnology

1. (2014) Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Xuebing Wu, David A Scott, and Andrea J Kriz, et al. Nature Biotechnology. 32:670–676. Link.
   Summary: In this study, four different sgRNAs and hemaglutinin-tagged dCas9 were used to target sequences in mouse embryonic stem cells. Sequence and genomic loci data of the off-target sites, acquired by ChiP-seq, suggests Cas9 requires PAM motif and 5 nucleotide seed for initial binding, but needs additional recognition for cleavage. Chromatin inhibits Cas9 binding, resulting in 70% off target effects occurring in gene-coding regions.
   
   
2. (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. John G Doench, Ella Hartenian, and Daniel B Graham et al. Nature Biotechnology, advanced online. Link.
   Summary: In this study, researchers examined six endogenous mouse and three endogenous human genes and made a pool of 1841 sgRNAs corresponding all the possible target sites in these nine genes. Using antibody staining and flow cytometry, the researchers quantitatively determined key features of highly-active sgRNAs and developed an online tool for targeting any gene of interest.
   
   

Nature Methods

1. Chemically defined generation of human cardiomyocytes

Summary: The researchers looked at a commonly used supplement in cell culture, B27, which has 27 different components, and identified only two that had any impact on cardiomyocyte differentiation: L-ascorbic acid 2-phosphate (a form of Vitamin C) and rice-derived recombinant human albumin. They used these two supplements in base RPMI medium to grow up TNNT2-positive cardiomyocytes with reported 95% efficency.

Nature Molecular Systems Biology

1. (year) Title. Author One, Author Two, and Author Three et al. Journal. Volume:pages. Link.
   Summary: Very short explanation of why this paper is relevant/ interesting.
   
   
2. (2011) Engineering a Photoactivated Caspase-7 for Rapid Induction of Apoptosis. Evan Mills, Xi Chen, Elizabeth Pham, Stanley Wong, and Kevin Truong et al. ACS Synthetic Biology, 1.3:75-82. Link.
   Summary: A group from University of Toronto developed a protein that causes rapid apotosis (cell death) of targeted cells.
   
   

Public Library of Science Biology (PLoS Biology)

Nothing super relevant.
Perhaps a bit relevant to Jan/David

1. (2014) Mitosis Gives a Brief Window of Opportunity for a Change in Gene Transcription Richard P. Halley-Stott, Jerome Jullien, Vincent Pasque et al. PLoSBio. 12(7). Link.
   Summary: Found that mitotic chromatin (which has most transcription factors temporarily displaced) is reprogrammed 100 times faster than interphase nuclei in post-nuclear transfer to amphibian oocytes. Genes pass through a transient phase of high responsiveness to reprogramming factors during mitosis which alters cell fates and is an opportunity for reprogramming to pluripotency. This may be a result of histone H2A deubiquitination.
   
   

Proceedings of the National Academy of Sciences

1. TBA

Science

1. (2014) H3K27me and PRC2 transmit a memory of repression across generations and during development. Laura J. Gaydos, Wenchao Wang2, Susan Strome. Science. 345 no. 6203 pp. 1515-1518 . Link.
   Summary: Attn. Jonah; Studied gene repression via methylation of H3K27me by PRC2. Generated embryos containing chromosomes with and without H3K27me. Without PRC2, H3K27me was transmitted to daughter chromatids through several rounds of cell division rather than the mosaic H3K27 pattern through embrygensis present in embryos with PRC2. Suggests that PRC2 represses X chromosomes in Caenorhabditis elegans germ cells. Shows that H3K27me and PRC2 both contribute to transmitting memory of repression across generations epigenetically.
   
   
2. (2014) Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling. Hans-Martin Herz, Marc Morgan, Xin Gao et al. Science. Vol. 345 no. 6200 pp. 1065-1070. Link.
   Summary: Established pathogenic histone H3K27M mutation in Drosophila. Noticed overexpression resembles PRC2 loss-of-function phenotypes. Examine other histone mutations in chromatin signaling pathways.
   
   
3. (2014) Chromatin state dynamics during blood formation. David Lara-Astiaso1, Assaf Weiner, Erika Lorenzo-Vivas et al. Journal. Vol. 345 no. 6199 pp. 943-949. Link.
   Summary: Attn. Meli'sa; High-sensitivity indexing-first chromatin immunoprecipitation approach to profile the dynamics of four chromatin modifications across 16 stages of hematopoietic differentiation developed. Paired with enhancer region studies to predict transcription factor network controlling chromatin dynamics programs in hematopoiesis.
   
   
4. (2014) Sequential histone-modifying activities determine the robustness of transdifferentiation. Steven Zuryn1, Arnaud Ahier, Manuela Portoso et al. Journal. Vol. 345 no. 6198 pp. 826-829 . Link.
   Summary: Attn. David; Explored efficiency of reprogramming events for cell conversion in vitro. Found conversion requires stepwise histone-modifying activities partitioned into fragmented phases of transdifferentiation through nuclear degradation of JMJD-3.1 and interactions with transcription factors at particular phases relevant in fate selection. Studied H3K27me3.
   
   

Miscellaneous Reviews and Media

Reviews

1. (2014) Chromatin dynamics in the regulation of cell fate allocation during early embryogenesis. Burton A, Torres-Padilla ME. Nat Rev Mol Cell Biol. doi: 10.1038/nrm3885. [Epub ahead of print] Link
   Summary: Good review for understanding how chromatin changes after one fertilized egg gives rise to many cells, and as subpopulations of these cells differentiate.
   
   
2. (2014) Synthetic biology in mammalian cells: next generation research tools and therapeutics. Lienert F, Lohmueller JJ, Garg A, Silver PA. Nat Rev Mol Cell Biol. 15:95-107. Link
   Summary: Attn. Dr. Haynes, Jonah; Pretty comprehensive teview on mammalian cel synthetic bio from the Silver lab. Includes chromatin engineering approaches (targeting of chromatin proteins fused with DNA binding domains). Good for figuring out what has not been explored yet & outstanding questions.
   
   
3. (2014) Synthetic therapeutic gene circuits in mammalian cells. Ye H, Fussenegger M. FEBS Letters. 588:2537-44. Link
   Summary: (another) review on mammalian synthetic circuits from the Fussenegger lab.
   
   
4. (2014) The Controversial Role of the Polycomb Group Proteins in Transcription and Cancer: How Much Do We Not Understand Polycomb Proteins? Scelfo A, Piunti A, Pasini D. FEBS J. doi: 10.1111/febs.13112. [Epub ahead of print]. Link
   Summary: Potentially game-changing information!
   
   
5. (2014) Epigenetic therapy of cancer with histone deacetylase inhibitors. Lakshmaiah KC, Jacob LA, Aparna S1, Lokanatha D, Saldanha SC. J Cancer Res Ther. 10:469-78. Link
   Summary: Useful for catching up on the state of the art in chromatin modification for cancer therapy. Also highlights adverse effects of HDAC therapy.
   
   

News

1. (2014) No One Should be Afraid of Synthetic Biology-Produced Vanilla. Haynes KA. Slate.com. Link
   
   
2. (2014) So Häagen-Dazs ‘says no to synbio’? Watson E. Food Navigator-USA.com. Link