Haynes:MamCultureMedia

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=Mammalian Culture Media=
=Mammalian Culture Media=
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==Transgenic Cells==
==Transgenic Cells==
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Revision as of 19:38, 27 October 2012

<- Back to Protocols

Mammalian Culture Media

"Wild type" stock cells (non-transgenic)

Cell line Incomplete media Supplements for complete media (required)
U2OS (adherent) McCoy's 5A 10% FBS, 1 % pen/strep
K-562 (suspension) Iscove's Modified Dulbecco's Medium (IMDM) 10% FBS, 1 % pen/strep


  • FBS = fetal bovine serum
  • FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
  • pen/strep = penicillin and streptomycin anti fungal supplement
  • Filter sterilize all media before use
  • Store all media at 4°C

Transgenic Cells

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