Haynes:MamCultureMedia: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 9: | Line 9: | ||
|- | |- | ||
| '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' | | '''Cell line''' || '''Incomplete media''' || '''Supplements for complete media (required)''' | ||
|- | |||
| HEK293 || DMEM high glucose || 10% FBS, 1 % pen/strep | |||
|- | |- | ||
| K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep | | K-562 (suspension) || Iscove's Modified Dulbecco's Medium (IMDM) || 10% FBS, 1 % pen/strep | ||
| Line 16: | Line 18: | ||
| U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep | | U2OS (adherent) || McCoy's 5A || 10% FBS, 1 % pen/strep | ||
|} | |} | ||
==Transgenic Cells== | ==Transgenic Cells== | ||
Revision as of 16:49, 27 October 2012
Mammalian Culture Media
"Wild type" stock cells (non-transgenic)
| Cell line | Incomplete media | Supplements for complete media (required) |
| HEK293 | DMEM high glucose | 10% FBS, 1 % pen/strep |
| K-562 (suspension) | Iscove's Modified Dulbecco's Medium (IMDM) | 10% FBS, 1 % pen/strep |
| SK-N-SH (adherent) | Eagle's Minimum Essential Medium (EMEM) | 10% FBS, 1 % pen/strep |
| U2OS (adherent) | McCoy's 5A | 10% FBS, 1 % pen/strep |
Transgenic Cells
| Cell Type | Genotype | Incomplete media | Supplements for complete media (required) | Antibiotic(s) for maintenance | Antibiotic(s) for selection |
| U2OS (adherent) | 126-1 | McCoy's 5A | 10% FBS, 1 % pen/strep | ## ug/mL puromycin | ## ug/mL puromycin |
Notes
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C
