Haynes:MamCultureMedia: Difference between revisions
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==Transgenic Cells== | |||
{| class="wikitable" | |||
|- | |||
| '''Cell Type''' || '''Genotype'' || '''Incomplete media''' || '''Supplements for complete media (required)''' || '''Antibiotic(s) for maintenance''' || '''Antibiotic(s) for selection''' | |||
|- | |||
| U2OS (adherent) || 126-1 || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin || ## ug/mL puromycin | |||
|} | |||
==Notes== | |||
* FBS = fetal bovine serum | * FBS = fetal bovine serum | ||
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* Filter sterilize all media before use | * Filter sterilize all media before use | ||
* Store all media at 4°C | * Store all media at 4°C | ||
</div> | </div> | ||
Revision as of 16:44, 27 October 2012
Mammalian Culture Media
"Wild type" stock cells (non-transgenic)
| Cell line | Incomplete media | Supplements for complete media (required) |
| K-562 (suspension) | Iscove's Modified Dulbecco's Medium (IMDM) | 10% FBS, 1 % pen/strep |
| SK-N-SH (adherent) | Eagle's Minimum Essential Medium (EMEM) | 10% FBS, 1 % pen/strep |
| U2OS (adherent) | McCoy's 5A | 10% FBS, 1 % pen/strep |
Transgenic Cells
| Cell Type | 'Genotype | Incomplete media | Supplements for complete media (required) | Antibiotic(s) for maintenance | Antibiotic(s) for selection |
| U2OS (adherent) | 126-1 | McCoy's 5A | 10% FBS, 1 % pen/strep | ## ug/mL puromycin | ## ug/mL puromycin |
Notes
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C
