Haynes:MamCultureMedia: Difference between revisions
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{| class="wikitable" cellspacing=5px | {| class="wikitable" cellspacing=5px | ||
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| '''Cell Type''' || '''Genotype''' || '''Medium''' || '''Supplements<br>(required)''' || '''Maintenance | | '''Cell Type''' || '''Genotype''' || '''Medium''' || '''Supplements<br>(required)''' || '''Maintenance<br>antibiotic(s)''' | ||
|- | |- | ||
| U2OS (adherent) || | | U2OS (adherent) || Flp-in T-REx || McCoy's 5A || 10% FBS, 1 % pen/strep || ## ug/mL puromycin | ||
|- | |||
| HEK 293 (adherent) || Flp-in T-REx || DMEM || 10% FBS, 1 % pen/strep || ## ug/mL puromycin | |||
|} | |} | ||
Revision as of 18:58, 19 January 2013
Mammalian Culture Media
"Wild type" stock cells (non-transgenic)
Cell line | Incomplete media | Supplements for complete media (required) |
HEK293 | DMEM high glucose | 10% FBS, 1% pen/strep |
K-562 (suspension) | Iscove's Modified Dulbecco's Medium (IMDM) | 10% FBS, 1% pen/strep |
SK-N-SH (adherent) | Eagle's Minimum Essential Medium (EMEM) | 10% FBS, 1% pen/strep |
U2OS (adherent) | McCoy's 5A | 10% FBS, 1% pen/strep |
Transgenic Cells
Cell Type | Genotype | Medium | Supplements (required) |
Maintenance antibiotic(s) |
U2OS (adherent) | Flp-in T-REx | McCoy's 5A | 10% FBS, 1 % pen/strep | ## ug/mL puromycin |
HEK 293 (adherent) | Flp-in T-REx | DMEM | 10% FBS, 1 % pen/strep | ## ug/mL puromycin |
Notes
- FBS = fetal bovine serum
- FBS-tf = tetracycline-free FBS (required to maintain the "off" state of dox-inducible transgenes)
- pen/strep = penicillin and streptomycin anti fungal supplement
- Filter sterilize all media before use
- Store all media at 4°C