Haynes:Protocols

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(Recipes)
(Recipes)
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'''Bromo-Blue/X-cyanol Loading Buffer, 10X''' {{hide|  
'''Bromo-Blue/X-cyanol Loading Buffer, 10X''' {{hide|  
-
Formula: [30% glycerol; 12.5 mg/ml bromophenol blue; 12.5 mg/ml xylene cyanol]
+
Formula: [30% glycerol; 12.5 mg/mL bromophenol blue; 12.5 mg/mL xylene cyanol]
Volume: 20 mL
Volume: 20 mL
-
* Caesin tryptone, 10 g
+
* 50% glycerol, 12 mL
-
* Yeast extract, 5 g
+
* Bromophenol blue, 250 mg
-
* NaCl, 5 g
+
* Xylene cyanol, 250 mg
-
* 1 N NaOH, 1 mL
+
 
-
Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
+
Bring up to total volume with dH<sub>2</sub>O.
}}
}}
Line 50: Line 50:
* NaCl, 5 g
* NaCl, 5 g
* 1 N NaOH, 1 mL
* 1 N NaOH, 1 mL
 +
Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
Dissolve in 1000 mL dH<sub>2</sub>O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.
}}
}}

Revision as of 14:47, 19 January 2012

link=Haynes_Lab
link=http://engineering.asu.edu/

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Protocols

BioBrick Assembly

Cell Culture: Bacteria

Cell Culture: Mammalian

  • Coming soon

Resources at OpenWetWare

  • Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.




Recipes

Bromo-Blue/X-cyanol Loading Buffer, 10X

Formula: [30% glycerol; 12.5 mg/mL bromophenol blue; 12.5 mg/mL xylene cyanol] Volume: 20 mL

  • 50% glycerol, 12 mL
  • Bromophenol blue, 250 mg
  • Xylene cyanol, 250 mg

Bring up to total volume with dH2O.

LB Broth (Lennox)

Volume: 1 L

  • Caesin tryptone, 10 g
  • Yeast extract, 5 g
  • NaCl, 5 g
  • 1 N NaOH, 1 mL

Dissolve in 1000 mL dH2O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.


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