Haynes:Protocols

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Protocols

BioBrick Assembly

• BioBrick assembly overview
• Making DNA parts for standardized assembly

Cell Culture: Bacteria

• Chemically competent cell prep

Cell Culture: Mammalian

• Coming soon

Resources at OpenWetWare

• Materials: useful buffer formulas and specific information on commonly used reagents, such as LB broth, ampicillin, restriction enzymes, etc.

Recipes

LB Broth (Lennox) [show][hide]

• Caesin tryptone, 10 g
• Yeast extract, 5 g
• NaCl, 5 g
• 1 N NaOH, 1 mL

Final volume: 1 L
Dissolve in 1000 mL dH₂O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.

LB Broth (Lennox) [show][hide]

• Caesin tryptone, 10 g
• Yeast extract, 5 g
• NaCl, 5 g
• 1 N NaOH, 1 mL

Dissolve in 1000 mL dH₂O. Autoclave to sterilize. Cool to room temperature before adding antibiotics. Shortcut: Use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone, yeast extract, and NaCl.

LB Broth (Lennox)
Caesin tryptone*	10 g
Yeast extract*	5 g
NaCl*	5 g
1 N NaOH	1 mL
	1000 mL

• Dissolve in 1000 mL dH₂O. Autoclave to sterilize.
• Cool to room temperature before adding antibiotics

* Note: Can use 25 g Acros LB Broth, Lennox (BP9722-500) granules instead of caesin tryptone,  yeast extract, and NaCl.