Haynes:Protocols - Revision history

Karmella Haynes: /* Flow Cytometry: Mammalian */

Karmella Haynes — Wed, 12 Jun 2013 00:08:54 GMT

Flow Cytometry: Mammalian

←Older revision		Revision as of 00:08, 12 June 2013
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-	==~~Flow Cytometry~~: Mammalian==	+	==Imaging: Mammalian==
		+
		+	'''Flow Cytometry'''
	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]		* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]
	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - how to detect cytoplasmic proteins using antibodies		* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - how to detect cytoplasmic proteins using antibodies
		+
		+	'''Microscopy'''
		+	* [[Haynes:Mamalian_IFC \| Immunocytology]] - staining fixed cells with antibodies

	==Protein==		==Protein==

Karmella Haynes — Wed, 12 Jun 2013 00:05:01 GMT

Flow Cytometry

←Older revision		Revision as of 00:05, 12 June 2013
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-	==Flow Cytometry==	+	==Flow Cytometry: Mammalian==
	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]		* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]
	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - how to detect cytoplasmic proteins using antibodies		* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - how to detect cytoplasmic proteins using antibodies

Karmella Haynes — Wed, 12 Jun 2013 00:04:10 GMT

Flow Cytometry

←Older revision		Revision as of 00:04, 12 June 2013
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	==Flow Cytometry==		==Flow Cytometry==
	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]		* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]
-	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - detect cytoplasmic proteins	+	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - how to detect cytoplasmic proteins using antibodies

	==Protein==		==Protein==

Karmella Haynes — Fri, 07 Jun 2013 15:35:33 GMT

Flow Cytometry

←Older revision		Revision as of 15:35, 7 June 2013
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	==Flow Cytometry==		==Flow Cytometry==
-	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]] ~~- no staining or fixing is required~~	+	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]]
	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - detect cytoplasmic proteins		* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - detect cytoplasmic proteins
-

	==Protein==		==Protein==

Karmella Haynes — Fri, 07 Jun 2013 15:35:12 GMT

←Older revision		Revision as of 15:35, 7 June 2013
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	* [[Haynes:Glycerol Stocks \| Glycerol Stocks]] - for long term -80°C storage		* [[Haynes:Glycerol Stocks \| Glycerol Stocks]] - for long term -80°C storage
	* [[Haynes:TransformationPlasmids \| Transformation of E. coli with plasmid DNA]]		* [[Haynes:TransformationPlasmids \| Transformation of E. coli with plasmid DNA]]
-


Line 35:		Line 34:
	* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine		* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine
	* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells		* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells
		+
		+
		+	==Flow Cytometry==
		+	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]] - no staining or fixing is required
		+	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - detect cytoplasmic proteins
		+

	==Protein==		==Protein==

Karmella Haynes — Fri, 07 Jun 2013 15:29:47 GMT

Karmella Haynes — Fri, 07 Jun 2013 15:29:14 GMT

Karmella Haynes — Tue, 14 May 2013 21:36:17 GMT

Cell Culture: Mammalian

←Older revision		Revision as of 21:36, 14 May 2013
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	* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine		* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine
	* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells		* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells
-

	==Protein==		==Protein==

Karmella Haynes — Mon, 13 May 2013 22:15:20 GMT

←Older revision		Revision as of 22:15, 13 May 2013
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	* [[Haynes:MamCultureMedia \| Media formulas]] - cell line-specific formulas		* [[Haynes:MamCultureMedia \| Media formulas]] - cell line-specific formulas
	* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine		* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine
		+	* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells

Karmella Haynes — Tue, 19 Mar 2013 23:58:08 GMT

Recipes

←Older revision		Revision as of 15:35, 7 June 2013
Line 28:		Line 28:
	* [[Haynes:Glycerol Stocks \| Glycerol Stocks]] - for long term -80°C storage		* [[Haynes:Glycerol Stocks \| Glycerol Stocks]] - for long term -80°C storage
	* [[Haynes:TransformationPlasmids \| Transformation of E. coli with plasmid DNA]]		* [[Haynes:TransformationPlasmids \| Transformation of E. coli with plasmid DNA]]
-


Line 35:		Line 34:
	* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine		* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine
	* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells		* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells
		+
		+
		+	==Flow Cytometry==
		+	* [[Haynes:FCMamalian_FluorGene \| Detecting Fluorescent Protein Expression]] - no staining or fixing is required
		+	* [[Haynes:ICCSaponin \| Intracellular Protein Staining with Saponin Permeabilization]] - detect cytoplasmic proteins
		+

	==Protein==		==Protein==

←Older revision		Revision as of 22:15, 13 May 2013
Line 34:		Line 34:
	* [[Haynes:MamCultureMedia \| Media formulas]] - cell line-specific formulas		* [[Haynes:MamCultureMedia \| Media formulas]] - cell line-specific formulas
	* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine		* [[Haynes:TransfectionPlasmid_Lipo \| Transfection - Lipofectamine]] - Transfection of plasmid DNA into cells with Lipofectamine
		+	* [[Haynes:SplittingCells \| Splitting Cells]] - passaging cells