Revision as of 17:20, 14 July 2014

Home Publications People Contact Protocols Lab Resources Personnel Calendar

BioBrick Collections

Chemicals, Etc.

Chemical Inventory Spreadsheet: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).
Compressed Gas: Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
Dry Ice: Located in PSG 106 at the DCB Stockroom

Literature

PubMed via ASU Libraries - navigating to PubMed through the ASU Libraries website will give you ASU password-protected access to scientific journals, free of charge

Ordering

Ordering Spreadsheet - CURRENT: Requests for consumables, DNA synthesis, and small instruments are listed here
Ordering Spreadsheets - ARCHIVED
- 2011-2013
SunRISE Ordering System: Authorized lab members can log-in to SunRISE to place orders
DNA Sequencing - SOLS DNA Lab [show][hide]

- Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
- Ordering Information
- Order Forms

DNA Sequencing - DNASU @Biodesign
- Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL
- Ordering Information
- Log in to Place an Order or Retrieve Results

Safety

PDF of the current, long version of the Haynes Lab SOP
Environmental Health & Safety at ASU
Biohazard & Chemical Waste Pick-up Request: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
Individual Training Record: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.

Shared Equipment

Sign-up Calendar
for all shared instruments

Burrrt Reynolds: -150°C Cryogenic Freezer
Model: Sanyo MDFC2156VANC
Location: ISTB4, room 231, linear equipment room
Status: Burrrt is up and running!

Lucille: Light Cycler 480
Model: Roche 5015278001
Location: ISTB4, room 231, south shared workspace
Status: Online

User technical information
Software downloads
Design a UPL assay - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green).

Plato: BioTek Plate Reader
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy
Location: ISTB4, room 231, south shared workspace
Status: Online

Wiki Editing

Basic Wiki editing tips - from the ASU BME100 course
Plasmid Validation Template Page - OWW notebook copy-paste code for DNA conc., restriction digests, etc.
Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php

@@ Line 2: / Line 2: @@
 [[Image:Hayneslab3.gif|link=Haynes_Lab]] [[Image:Asu_logo_3.gif|right|link=http://engineering.asu.edu/]]<br>
 &nbsp;&nbsp;[[Haynes_Lab | <font face="trebuchet ms" style="color:#000000"> '''Home''' </font>]] &nbsp;&nbsp;&nbsp;
+[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
+[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
 [[Haynes:Contact | <font face="trebuchet ms" style="color:#000000"> '''Contact''' </font>]] &nbsp;&nbsp;&nbsp;
 [[Haynes:Protocols | <font face="trebuchet ms" style="color:#000000"> '''Protocols''' </font>]] &nbsp;&nbsp;&nbsp;
-[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Technical Resources''' </font>]] &nbsp;&nbsp;&nbsp;
+[[Haynes:TechResources | <font face="trebuchet ms" style="color:#000000"> '''Lab Resources''' </font>]] &nbsp;&nbsp;&nbsp;
-[[Haynes:Lab Members | <font face="trebuchet ms" style="color:#000000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;
+[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Personnel''' </font>]] &nbsp;&nbsp;&nbsp;
-[[Haynes:Publications | <font face="trebuchet ms" style="color:#000000"> '''Publications''' </font>]] &nbsp;&nbsp;&nbsp;
+[[Haynes:Calendar | <font face="trebuchet ms" style="color:#000000"> '''Calendar''' </font>]]
-[[Haynes:Research | <font face="trebuchet ms" style="color:#000000"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;
-[[Haynes:Internal | <font face="trebuchet ms" style="color:#000000"> '''Internal Info''' </font>]]
 </div><br>
-=Technical Resources=
+<div style="width: 1000px">
+=BioBrick Collections=
+* [https://benchling.com/hayneslab Benchling - Haynes Lab Parts & Plasmids]
+* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab iGEM Registry - Haynes Lab Parts]
+* [https://docs.google.com/spreadsheet/ccc?key=0AghpgNgJLx-ldFVkeDFnYjBYMVFHRm5Ta0ZXaVVGZEE Dr. Haynes' parts and vectors]
+* [https://docs.google.com/spreadsheet/ccc?key=0As4aQtxfHElzdG8tYllENlE5OEhnRDNCN0ZoY0xvRnc Dr. Haynes' primers]
+* [https://docs.google.com/spreadsheet/ccc?key=0Avv2LLPPodHAdDhDR1hFUXlvSFZleGd2RWhrcW03QXc#gid=2 René's primers, oligos, and parts]
+* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0Aj7J45qrNRPzdFp5c201WEwxdDlLcGgzQmsxVnBWS3c#gid=0 Brady's Parts]
+<br><br>
-==BioBrick Collections==
+=Chemicals, Etc.=
-* [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=lab&group=Haynes%20Lab Haynes Lab Parts]
+* [https://docs.google.com/spreadsheet/ccc?key=0AoCP2OrURah2dDJtal9Bc1VDbUNvd0xsRDJXNG03VEE&hl=en_US#gid=0 Chemical Inventory Spreadsheet]: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).<br>
+* '''Compressed Gas''': Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
+* '''Dry Ice''': Located in PSG 106 at the DCB Stockroom
 <br>
-==Chemicals==
+=Literature=
-* [https://docs.google.com/spreadsheet/ccc?key=0AoCP2OrURah2dDJtal9Bc1VDbUNvd0xsRDJXNG03VEE&hl=en_US#gid=0 Chemical Inventory Spreadsheet]: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).<br>
+* [https://login.ezproxy1.lib.asu.edu/login?url=http://www.ncbi.nlm.nih.gov/sites/entrez?otool=azuazslib PubMed via ASU Libraries] - navigating to PubMed through the [http://lib.asu.edu/ ASU Libraries website] will give you ASU password-protected access to scientific journals, free of charge
 <br>
-==Ordering==
+=Ordering=
-* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHkySmt3TTUyLTBMUGlsSk5zdGYwb1E Ordering Spreadsheet]: Requests for consumables, DNA synthesis, and small instruments are listed here
+* [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHNPcDlwb2VteXBhMnJmVWNXaXVoTmc&usp=sharing Ordering Spreadsheet - CURRENT]: Requests for consumables, DNA synthesis, and small instruments are listed here
+* '''Ordering Spreadsheets - ARCHIVED'''
+** [https://docs.google.com/a/asu.edu/spreadsheet/ccc?key=0AghpgNgJLx-ldHkySmt3TTUyLTBMUGlsSk5zdGYwb1E 2011-2013]
 * [http://cfo.asu.edu/purchasing-sunrise SunRISE Ordering System]: Authorized lab members can log-in to SunRISE to place orders
-* '''DNA Sequencing: SOLS DNA Lab''':
+* '''DNA Sequencing - SOLS DNA Lab''' {{hide|
+** Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
 ** [http://sols.asu.edu/labs/dna_lab/serv.php Ordering Information]
 ** [http://sols.asu.edu/labs/dna_lab/forms.php Order Forms]
-* '''DNA Sequencing: DNASU''':
+}}
+* '''DNA Sequencing - DNASU @Biodesign'''
+** Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL
 ** [http://sequencing.biodesign.asu.edu/ Ordering Information]
-** [http://dnatools.biodesign.asu.edu/ Login/ Create Account]
+** [http://dnatools.biodesign.asu.edu/cgi-bin/dna/password.cgi Log in to Place an Order or Retrieve Results]
+<br><br>
+=Safety=
+* PDF of the current, long version of the [http://openwetware.org/images/c/cf/Haynes_Lab_SOP_2014.pdf Haynes Lab SOP]
+* [https://cfo.asu.edu/ehs Environmental Health & Safety at ASU]
+* [http://ehsaweb.asu.edu/ Biohazard & Chemical Waste Pick-up Request]: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
+* [http://ehsaweb.asu.edu/ Individual Training Record]: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
 <br><br>
-==Shared Equipment==
+=Shared Equipment=
+[[Haynes:Equipment Signup | Sign-up Calendar]]<br>
+for all shared instruments
 '''Burrrt Reynolds: -150°C Cryogenic Freezer'''<br>
@@ Line 51: / Line 78: @@
 Location: ISTB4, room 231, south shared workspace<br>
 Status: Online<br>
-* [http://www.google.com/calendar/embed?src=asu.edu_7s7m81pvth8183tnlnmim9a7io%40group.calendar.google.com&ctz=America/Phoenix Sign-up calendar]: It is '''mandatory''' that you sign up before using the Light Cycler 480. E-mail [[User:Karmella Haynes|Karmella Haynes]] to gain access to the Google calendar.
 * [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?&id=htc_060000 User technical information]
 * [http://www.roche-applied-science.com/sis/rtpcr/htc/index.jsp?id=htc_070400 Software downloads]
@@ Line 61: / Line 87: @@
 Location: ISTB4, room 231, south shared workspace<br>
 Status: Online<br>
+=Wiki Editing=
+* [http://openwetware.org/wiki/BME100_f2013:WikiHelp Basic Wiki editing tips] - from the ASU BME100 course
+* [[Haynes:WikiTemp_NBplasmids | Plasmid Validation Template Page]] - OWW notebook copy-paste code for DNA conc., restriction digests, etc.
+* Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php
+</div>

Haynes:TechResources: Difference between revisions

Revision as of 17:20, 14 July 2014

Contents

BioBrick Collections

Chemicals, Etc.

Literature

Ordering

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Shared Equipment

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