Haynes:TechResources: Difference between revisions
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* '''DNA Sequencing - SOLS DNA Lab''' | * '''DNA Sequencing - SOLS DNA Lab''' | ||
** Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube | ** Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube | ||
** [https://sols.asu.edu/resources/core-laboratories] | ** [https://sols.asu.edu/resources/core-laboratories Submission Information] | ||
** [https://sols.asu.edu/sites/default/files/undergraduate/pdf/DNAsequencing_form1.pdf Order Form] | ** [https://sols.asu.edu/sites/default/files/undergraduate/pdf/DNAsequencing_form1.pdf Order Form] | ||
** Cloest drop-off: Biodesign building, go down stairs to lower level, turn left at wall, enter hallway, go to end, turn right and find the sample freezer (on the way to the restrooms) | |||
* '''DNA Sequencing - DNASU @Biodesign''' | * '''DNA Sequencing - DNASU @Biodesign''' | ||
** Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL | ** Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL | ||
Revision as of 15:22, 27 October 2015
BioBrick Collections
- Benchling - Haynes Lab Parts & Plasmids
- iGEM Registry - Haynes Lab Parts
- Dr. Haynes' parts and vectors
- Dr. Haynes' primers
- René's primers, oligos, and parts
- Brady's Parts
- Ben's parts
Chemicals, Etc.
- Chemical Inventory Spreadsheet: This Google Docs spreadsheet lists all of the chemicals in the Haynes Lab inventory, plus safety precautions and links to the Material Safety Data Sheets (MSDS).
- DCB Chemical Stockroom: Located at Physical Sciences Building G-wing (PSG), "Bateman Storage", ground floor - https://maps.asu.edu/?id=120&mrkIid=62979
- Compressed Gas: Contact ASU Stores - Gas Services at 5-6546 or gascylinders (at asu dot edu)
- Dry Ice: Located in PSG 106 at the DCB Chemical Stockroom
Facilities, Maintenance & Repairs
- ISTB4 Building Manager - Frank N. Clark (as of 2014)
- Work Orders - submit work orders through this page: http://webtma.asu.edu/ASUServiceRequest/Default.aspx
- Disposal of working instruments and equipment: submit pickup request through ASU Surplus https://webapp3.asu.edu/spms/public.aspx
- Repairs: small instruments - submit work orders to the Instrument & Prototype Machine Shop through this page: https://fultonapps.asu.edu/shop/
Literature
- PubMed via ASU Libraries - navigating to PubMed through the ASU Libraries website will give you ASU password-protected access to scientific journals, free of charge
Ordering & Shipping
- Spreadsheet - CURRENT: Requests for consumables, DNA synthesis, and small instruments are listed here
- Ordering Spreadsheets - ARCHIVED (For information purposes only. Do not edit these)
- SunRISE Ordering System: Authorized lab members can log-in to SunRISE to place orders
- DNA Sequencing - SOLS DNA Lab
- Sample submission: 20 ng (~20 pmol) primer, 200 ng plasmid DNA, 6 μL total volume in a single tube
- Submission Information
- Order Form
- Cloest drop-off: Biodesign building, go down stairs to lower level, turn left at wall, enter hallway, go to end, turn right and find the sample freezer (on the way to the restrooms)
- DNA Sequencing - DNASU @Biodesign
- Sample submission: 10 ng primer (~10 pmol = 1μL of 10μM primer) plus 200 ng plasmid DNA in 10 μL
- Ordering Information
- Log in to Place an Order or Retrieve Results
- Central Receiving - where large items/ equipment arrives. Call Louie: 480-965-1083
Office Supplies & Poster Printing
- Poster printing
- ASU Vislab - [1]
Safety
- Standard Operating Procedures (SOP) - PDF of the current SOP. Read this immediately after joining the Haynes lab.
- Environmental Health & Safety at ASU
- Biohazard & Chemical Waste Pick-up Request: log in at the EHS Assistant page to request biohazard waste pick-up. Use the "Chemical Waste Pick-up" link, click the "i" button next to Name of PI, and search for Haynes. For lab location, search for "Interdisciplinary Science and Technology Building, 231" (BIO for biohazard, CHEM for chemicals)
- Individual Training Record: To check your individual safety training record, log in at the EHS Assistant page. Click the "Individual Training Record" link and then enter your ASU ID.
Sign-up Calendar
for all shared instruments
Burrrt Reynolds: -150°C Cryogenic Freezer
Model: Sanyo MDFC2156VANC
Location: ISTB4, room 231, linear equipment room
Status: Burrrt is up and running!
Lucille: Light Cycler 480
Model: Roche 5015278001
Location: ISTB4, room 231, south shared workspace
Status: Online
- User technical information
- Software downloads
- Design a UPL assay - UPL probes provide the specificity of hydrolysis probe assays (e.g., TaqMan), at a much lower cost (comparable to SYBR Green).
Plato: BioTek Plate Reader
Model: Synergy 2; luminescence, fluorescence, absorbance; Take3 nucleic acid spectroscopy
Location: ISTB4, room 231, south shared workspace
Status: Online
Wiki Editing
- Basic Wiki editing tips - from the ASU BME100 course
- Plasmid Validation Template Page - OWW notebook copy-paste code for DNA conc., restriction digests, etc.
- Excel to Wiki table converter: here's a neat tool that let's you paste data from Excel into a form and covert it into Wiki code by clicking a button. http://excel2wiki.net/wikipedia.php
