Haynes:Volunteer Duties

All volunteer prep work (reagents, media, autoclaving plastics, etc.) must be done in the designated volunteer work space (the shared chemicals bay/ area near Dr. Haynes' bench, far South end of the lab) ONLY.

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Level A

Eligibility: no molecular biology experience required, safety training and ISAAC access required, ASU undergrads (high school students may assist UG volunteers)

Lab Supplies - Shipping and Mail

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4 231 and ECG 344
• Tasks
  • Part 1: Go to ISTB4 273. Check the blue OUTBOX on Dr. Haynes' bench for any outgoing mail. Open Trello on the lab computer and look for any packages that need to be picked up (Labeled “Pick-up” under TO-DO). If there are any packages to pick up, get the black cart. Use the ISTB4 main elevator to exit the building with the cart.
  • Part 2: Go to ECG 344. Go to 344A to pick up packages (if any) and sign the package log on the clipboard. Go to the mail room and drop off outgoing mail (if any). Pick up mail from Karmella Haynes’ mail box.
  • Part 3: Return to ISTB4 273. Drop off packages at Dr. Haynes’ lab bench. Do NOT open or unpack the box(es). Drop off mail in the INBOX. Open Trello on the lab computer and move the relevant pick-up card to the DONE section.
• Time = ~1 hour

Lab Space - Waste Prep

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231
• Tasks
  • Part 1, option A: Check the biohazard waste bags at the end of each lab bench. If there are no ½ full or totally full bags, move on to option B. Take ONE full bag, remove the bag from the stand, twist the bag closed, and use lab tape to seal the closure. Move on to Task part 2.
  • Part 1, option B: Check the biohazard waste bag in the tissue culture room (under the hood). If there is no ½ full or totally full bag, then you are done with this assignment and you can leave. Take ONE full bag, remove the bag from the stand, twist the bag closed, and use lab tape to seal the closure. Move on to Part 2.
  • Part 2: Place the largest autoclave bin onto the black roll-away cart. Put the biohazard waste bag into the bin. Take a pair of orange safety gloves. Take the bag/bin/cart into the freight elevator and up to the 3rd floor autoclave. If there are any hot items in the autoclave, carefully remove them and place them on a nearby table. Login as “Haynes” on the autoclave. Place the bag + bin into the autoclave, close the door, and run any “dry” or “waste” program. NEVER place a bag into the autoclave without it sitting inside an autoclave bin!
  • Part 3: After the cycle has ended (usually 40 min), put on a pair of orange safety gloves, retrieve the autoclaved material, and bring it back to the Haynes lab. Place the autoclaved material into its proper bin. If it came from the lab, place it into the bin by the shaking incubator. If it came from the TC room, pace it into that bin. Do not mix general lab and TC room waste.
• Time = ~ 1 hour

Lab Space - Waste Pickup

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231
• Tasks
  • Part 1: Go to the empty box disposal area. Dump any packaging/ stuffing (bubble wrap, peanuts, paper) into one of the trash bins in the lab. Break down the cardboard box so that it lays flat (remove or cut tape if necessary). Take the broken-down boxes to the LER (by the freight elevator) and place them in the large blue roll-away bin.
  • Part 2: Go to the fume hood in the lab and check for bottles that have a paper Biohazard waste tag hanging on it. These are ready for pick-up. Go to a lab computer and log-in to EH&S Assistant: http://ehsaweb.asu.edu/. Click Chemical Waste Pickup Request. Complete the form. Karmella Haynes is the PI and Contact. Set “Lab/room” to 231, CHEM. In the Comments box, type “Fume hood:” followed by each bottle and its contents.
  • Part 3: (1) Go to the big orange or grey biohazard waste bin the lab. Open the lid to check for any bags. (2) Go into the tissue culture room. Check the big orange or grey waste bin for any bags. If there are ANY there, these are ready for pick-up. Go to the computer and log-in to EH&S Assistant: http://ehsaweb.asu.edu/. Click Chemical Waste Pickup Request. Complete the form. Karmella Haynes is the PI and Contact. Set “Lab/Location” to 231, BIO. In the Comments box, type “Biohazard waste bin(s):” followed by a description of which bins are full.
• Time = ~1 hour

Equipment - Freezers

• Frequency: Usually once a semester, on a day designated by Dr. Haynes
• Location(s): ISTB4, 231
• Tasks
  • Part 1: Check the -20°C freezer for any excessive build-up of ice on the top, walls, and shelves. Notify Dr. Haynes of excessive build-up. If there is no build-up, your task is done. If there is, proceed to part 2.
  • Part 2: Wait for Dr. Haynes to instruct the other lab members to clear out their items.On the day designated by Dr. Haynes, after -20C freezer is cleared out by the lab members, open the freezer and scrape out the ice build-up with a provided spatula and/or other tools. Collect fallen ice into a bin/ bucket and dump the ice in the sink.
• Time = ~1 hour

Level B

Eligibility: Success of lab research projects relies heavily on the quality of this work. Therefore, requirements include: Molecular biology or chemistry experience (7 days of canned PCR in BME 100 is not sufficient, sorry) OR at least one full active semester of Level A volunteer experience; safety training and ISAAC access required, ASU undergrads (high school students may assist/ shadow UG volunteers)

Safety Note: The tasks require a lab coat and gloves, at minimum. Wear safety goggles when handling reagents.

Lab reagents - 70% ethanol

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231
• Task(s)
  • Part 1: Check the 70% etOH carboy. If the carboy is more than 1/8 full, do not refill it. If it is nearly empty, pour the small remaining amount of 1x TAE into a 500 mL glass bottle and label it "70% etOH" Next, follow the instructions written on the carboy. You must use a fresh, unopened, full gallon of 95% etOH. The gallons of 95% etOH (marked "Haynes Lab") are in the tall yellow Flammables cabinet back in the LER. If there is not enough 95% etOH there, proceed to Part 2.
  • Part 2: Send an e-mail to Dr. Haynes to request an order for two gallons of 95% etOH.
• Time = ~1 hour

Lab reagents - TAE buffer

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231
• Task(s)
  • Part 1: Check the 1x TAE carboy. If the carboy is more than 1/8 full, do not refill it. If it is nearly empty, pour the small remaining amount of 1x TAE into a 500 mL glass bottle and label it "1x TAE." Next, follow the instructions written on the carboy - use the 50x TAE to refill it to the proper level. Do NOT fill it all the way up to 8 liters! If there is not enough 50x TAE, proceed to Part 2.
  • Part 2: Make Tris-acetate-EDTA (TAE) Buffer, 50x
• Time = ~1 hour

Lab reagents - agar plates

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231

Task(s)
	Part 1: Go the 4°C cold room in the LER. Check the black bin(s), marked Haynes for agar plates. Quickly count and estimate the number of remaining plates of each type (e.g., Amp, LB plain, etc.). Inspect each stack's date. Check to see if there is any mold/ weird growth. If any plates are moldy and/or more than three months old, proceed to Part 2. If there are less than 20 good plates of any type, proceed to Part 3.
	Part 2: Retrieve one orange AND one clear biohazard waste bag from the lab. Make a double bag, with the orange bag inside the clear bag. Bring it to the cold room. Put plates that are more than 3 months old and moldy plates into the double-bag. Bring the bag to the lab. Twist the bag closed and seal it with tape. Add a tape-label that says "please autoclave". Place the bag next to (not inside) the large biohazard bin. DO NOT do anything with plates that are NOT in the black Haynes bins!!! If anyone else's plates are moldy, the owner is responsible for discarding them.
	Part 3: Make Agar Plates
	Time = ~1 hour

Lab reagents - liquid medium

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231

Task(s)
	Part 1: Go to the shared chemicals shelf in the lab and look for the LB Liquid media. Check the bottles for any cloudy growth. If any of the bottles has cloudiness or mold, proceed to Part 2. If there are two or fewer good bottles left, proceed to Part 3.
	Part 2: To discard of contaminated LB liquid medium, take the bottle to the sink. Add about 1/20th volume of 50% bleach from the squirt bottle directly into the contaminated medium. Close the cap and swirl to mix thoroughly. Pour the medium + bleach into the proper container in the fume hood. DO NOT pour it down the sink or floor drain.
	Part 3: Check the shared chemicals shelf for the reagents required to make LB liquid medium: Acros LB Broth, Lennox (BP9722-500) and 1 N NaOH. If anything is missing or low, notify Dr. Haynes via e-mail. If there is enough, proceed to Part 4.
	Part 4: Make LB Liquid Medium in the 200 mL rectangular glass bottles. Fill each bottle to exactly 200 mL.
	Time = ~2 hours

Glassware & Plasticware

• Frequency: On the volunteer’s designated day, at least once a week
• Location(s): ISTB4, 231
• Task(s)
  • Part 1: Go to the Haynes lab sink and look for dirty glassware/ plasticware in the plastic bin. If there are any items there, proceed to Part 2. If there are clean dry items on the drying rack, proceed to Part 3. Note: Do not collect dirty glassware/ plasticware from the lab benches. It is every lab member's responsibility to properly empty their used items and place them into the bin.
  • Part 2: Wash each item with diluted lab soap and tap water (the faucet that has both "hot" and "cold" handles on each side) and an appropriate white fluffy washer. Rinse away the soap with tap water, then rinse away the tap water with deionized water (the faucet with the push-down handle on top). Place the clean item on the drying rack on the wall above the sink. If there is a bottle cap, place it to dry on the ledge of the drying rack. Let the items dry for at least one day.
  • Part 3: After the glassware has dried, retrieve an autoclave bin and place it on the black Haynes lab cart. If the dried items have open tops without lids (beakers, flasks, graduated cylinders, etc.), retrieve a piece of aluminum foil from the media prep shelf and completely (and neatly) cover the opening with foil. Place a piece of autoclave tape on the foil. If the dried items include bottle with caps, loosely screw the cap onto the bottle and place a piece of autoclave tape on the cap. Proceed to Part 4.
  • Part 4: Use the LER freight elevator to take the items up to the third floor autoclave. If there are any hot items in the autoclave, carefully remove them and place them on a nearby table. Login as “Haynes” on the autoclave. Place the tray of items into the autoclave, close the door, and run any “dry” program.
  • Part 5: After the cycle has ended (usually 40 min), put on a pair of orange safety gloves, retrieve the autoclaved material, and bring it back to the Haynes lab. Place the autoclaved items onto their proper shelves.
• Time = 2 consecutive days, ~1 hour each day

Sterile Tubes

• Frequency: On the volunteer’s designated day, about every other week
• Location(s): ISTB4, 231

Tasks
	Part 1: Go to the shelf where the sterile tubes are stored and check for empty containers. If there are empty containers, proceed to Part 2.
	Part 2: Take the empty containers and some tubes back to the volunteers media prep area... If any 1.5 mL tube containers are empty, retrieve a bag of 1.5 mL tubes from its storage drawer (located in the labeled cabinet drawers under the research benche) and bring it back to the media prep area. If any 0.5 mL tube containers are empty, retrieve a bag of 0.5 mL tubes from its storage drawer (located in the labeled cabinet drawers under the research benche) and bring it back to the media prep area.
rowspan=2		Part 3: Carefully fill each empty sterile tube container with the appropriate-sized tubes. Close the lid(s) and seal it with a piece of autoclave tape. Write the current date (mm/dd/yy) on the tape. Place the box(es) into an autoclave tray, and place the filled tray onto the black Haynes Lab cart. If there are still empty containers, use more bags of tubes as necessary to fill all empty containers. If you run out of tubes, contact Dr. Haynes before the end of the day. Proceed with Part 4.
	Part 4: Use the LER freight elevator to take the items up to the third floor autoclave. If there are any hot items in the autoclave, carefully remove them and place them on a nearby table. Login as “Haynes” on the autoclave. Place the tray of items into the autoclave, close the door, and run any “dry” program.
	Part 5: After the cycle has ended (usually 40 min), put on a pair of orange safety gloves, retrieve the autoclaved material, and bring it back to the Haynes lab. Place the autoclaved items onto the appropriate storage area.
	Time = ~1-2 hours