Haynes:WikiTemp NBplasmids: Difference between revisions
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| bgcolor=#cfcfcf | Reagent || Volume | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br> | | rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br> | ||
| rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] |
Revision as of 07:37, 3 September 2013
DNA Concentration Data
Plasmid | OD260 | OD260/280 | ng/μL |
1. Plasmid 1 | --- | --- | --- |
2. Plasmid 2 | --- | --- | --- |
Restriction Digest Table
- Check plasmid minipreps with EcoRI/PstI digests
Reagent | Volume | Expected: 1. Plasmid 1 = insert size, vector size 2. Plasmid 2 = insert size, vector size |
15 μL/lane; 1% agarose; Ladder |
DNA(plasmid) | 3.0 μL | ||
10X buffer | 1.5 | ||
EcoRI | 1.0 | ||
PstI | 1.0 | ||
dH2O | 8.5 | ||
15 μL --> 37°C/ 15 min. |