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|
Line 21: |
Line 21: |
| | Plasmid || OD260 || OD260/280 || ng/μL | | | Plasmid || OD260 || OD260/280 || ng/μL |
| |- | | |- |
| | 1. Plasmid 1 || --- || --- || --- | | | 1. Plasmid 1 || 0.034 || 1.8 || 34.227 |
| |- | | |- |
| | 2. Plasmid 2 || --- || --- || --- | | | 2. Plasmid 2 || 0.025 || 1.953 || 24.576 |
| |} | | |} |
|
| |
|
|
| |
| '''Restriction Digest Table'''<br>
| |
| * Checked plasmid minipreps with EcoRI/PstI digests
| |
|
| |
| {| {{table}} border="1" cellspacing="3"
| |
| <!-- Editing: the coding for this table is a bit more advanced. -->
| |
| <!-- valign="top" aligns all the text in the first row to the top. -->
| |
| <!-- The | symbols on the next two lines start new cells in the same row. This does the same thing as ||, but you have to use | on new lines to set formatting for cells. -->
| |
| <!-- bgcolor=#cfcfcf *colors* the *background* of the Reagent and Volume cells grey. -->
| |
| <!-- The next two "rowspan=7" cells span all 7 rows in the table so that they can fit the "Expected" list and a gel image. rowspan is how you create merged rows in Wiki code. -->
| |
| <!-- Replace Plasmid 1 and 2 with the names of your plasmids. Replace insert size and vector size with appropriate information for your plasmids. If you only have one Plasmid, delete all the text for Plasmid 2
| |
| <!-- After you upload your gel image to OWW, replace "GelImage.jpg" with the name of your image file -->
| |
| <!-- is ASCII code for an invisible space. -->
| |
| |- valign="top"
| |
| | bgcolor=#cfcfcf | Reagent
| |
| | bgcolor=#cfcfcf | Volume
| |
| | rowspan="7" | <u>Expected:</u><br>1. Plasmid 1 = insert size, vector size<br>2. Plasmid 2 = insert size, vector size<br>
| |
| | rowspan="7" | [[Image:GelImage.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| |
| |-
| |
| | DNA(plasmid) || 3.0 μL
| |
| |-
| |
| | 10X buffer || 1.5
| |
| |-
| |
| | EcoRI || 1.0
| |
| |-
| |
| | PstI || 1.0
| |
| |-
| |
| | dH<sub>2</sub>O || 8.5
| |
| |-
| |
| | || 15 μL --> 37°C/ 15 min.
| |
| |}
| |
|
| |
|
| |
| '''DNA Sequencing Samples'''
| |
| <!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples -->
| |
| <!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below -->
| |
| * Submitted to DNASU on: 'date'
| |
| * Order Number:
| |
| <!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. -->
| |
| # Plasmid 1 - forward primer 'name'
| |
| # Plasmid 1 - reverse primer 'name'
| |
| # Plasmid 2 - forward primer 'name'
| |
| # Plasmid 2 - reverse primer 'name'
| |
|
| |
|
| <!--TEMPLATE CODE--> | | <!--TEMPLATE CODE--> |